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Generation of a heterozygous p53 R249S mutant human embryonic stem cell line by TALEN-mediated genome editing.

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收录情况： ◇ SCIE

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机构： [1]Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA [2]Department of Endocrinology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, PR China [3]Department of Musculoskeletal Oncology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, PR China [4]Department of Pathology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, PR China [5]Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, PR China [6]The University of Texas, MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Houston, TX 77030, USA [7]Guangdong Research Center of Metabolic Diseases of Integrated Western and Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, PR China [8]Women's Health Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA [9]Division of Hematology and Oncology, Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan

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As one of the most essential genome guardians, p53 and its mutants have been suggested associated with many types of cancers. Many p53 mutants function induce unique phenotypes, including carcinogenesis, metastasis, and drug resistance. The p53(R249S) mutation is the most prevalent and specific mutation associated with liver cancer development. Here, we demonstrate the generation of a heterozygous p53(R249S) mutation in the H9 human embryonic stem cell line using TALEN-mediated genome editing. The generated cell line maintains a normal karyotype, a pluripotent state and the in vivo capacity to develop a teratoma containing all three germ layer tissues. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

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出版当年[2018]版：

大类 | 2 区生物

小类 | 2 区生物工程与应用微生物 3 区细胞与组织工程 3 区细胞生物学

最新[2025]版：

大类 | 4 区医学

小类 | 4 区生物工程与应用微生物 4 区细胞与组织工程 4 区细胞生物学

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出版当年[2017]版：

Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Q2 CELL & TISSUE ENGINEERING Q2 CELL BIOLOGY

最新[2023]版：

Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Q4 CELL & TISSUE ENGINEERING Q4 CELL BIOLOGY

影响因子： 0.8 最新[2023版] 1.1 最新五年平均 3.902 出版当年[2017版] 4.127 出版当年五年平均 3.963 出版前一年[2016版] 3.929 出版后一年[2018版]

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第一作者机构： [1]Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA [2]Department of Endocrinology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, PR China

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