机构：[1]CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, China,[2]Foshan Hospital of Traditional Chinese Medicine, Foshan, Guangdong, China,[3]Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China
Defining and loading of immunogenic and safe cancer antigens remain a major challenge for designing dendritic cell (DC)-based cancer vaccines. In this study, we defined a prototype strategy of using DC-based vaccines pulsed with placenta-derived heat shock protein gp96 to induces anti-tumor T cell responses. Placental gp96 was efficiently taken up by CD11c+ bone marrow-derived DCs (BMDCs) and resulted in moderate BMDC maturation. Splenocytes and cytotoxic T cells (CTLs) generated with mouse BMDCs pulsed with placental gp96 specifically lysed B16 melanoma and LLC lung carcinoma cells. In both transplantable melanoma and lung carcinoma mice models, immunization with placental gp96-stimulated BMDCs led to a significant decrease in tumor growth and mouse mortality with respect to mice treated with liver gp96-pulsed BMDCs or placental gp96 alone. This vaccine induced strong cross-reactive tumor-specific T cell responses. Our results revealed that DCs pulsed with placenta-derived gp96 represent an effective immunotherapy to induce tumor-reactive immune responses, possibly via loading DCs with its associated carcinoembryonic antigens.
基金：
Songdong Meng (SM) received the
following awards: National Key R&D Program
(grant number: 2016YFC1303402) http://www.
most.gov.cn/; Strategic Priority Research Program
of the Chinese Academy of Sciences (grant
number: XDPB030404) http://www.cas.cn/; One
Belt and One Road International Science and
Technology Cooperation of Chinese Academy of
Sciences (grant number: 153211KYSB20170001) http://www.cas.cn/; National Natural Science
Foundation of China (grant numbers:
81761128002, 81621091, 81471960, 81672815)
http://www.nsfc.gov.cn/.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|3 区生物
小类|3 区综合性期刊
最新[2025]版：
大类|3 区综合性期刊
小类|3 区综合性期刊
第一作者：
第一作者机构：[1]CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, China,
通讯作者：
通讯机构：[1]CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, China,[3]Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China
