机构：[1]Healthy Aquaculture Laboratory, College of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong, China,[2]Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong, China,[3]Department of Traditional Chinese Medicine, Renmin Hospital of Wuhan University, Wuhan, Hubei, China
Koumine is a component of the Chinese medicinal herb Gelsemium elegans and is toxic to vertebrates. We used the ciliate Tetrahymena thermophila as a model to evaluate the toxic effects of this indole alkaloid in eukaryotic microorganisms. Koumine inhibited T. thermophila growth and viability in a dose-dependent manner. Moreover, this drug produced oxidative stress in T. thermophila cells and expressions of antioxidant enzymes were significantly elevated at high koumine levels (p < 0.05). Koumine also caused significant levels of apoptosis (p < 0.05) and induced DNA damage in a dose-dependent manner. Mitophagic vacuoles were present in cells indicating induction of autophagy by this drug. Expression of ATG7, MTT2/4, CYP1 and HSP70 as well as the MAP kinase pathway gene MPK1 and MPK3 were significantly altered after exposed to koumine. This study represents a preliminary toxicological evaluation of koumine in the single celled eukaryote T. thermophila.
基金：
This study was supported by the
earmarked fund for China Agriculture Research
System(CARS-45) to JZ. The funders had no role
in study design, data collection and analysis,
decision to publish, or preparation of the
manuscript.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|3 区生物
小类|3 区综合性期刊
最新[2025]版：
大类|3 区综合性期刊
小类|3 区综合性期刊
第一作者：
第一作者机构：[1]Healthy Aquaculture Laboratory, College of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong, China,[2]Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong, China,
通讯作者：
通讯机构：[1]Healthy Aquaculture Laboratory, College of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong, China,[2]Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong, China,
推荐引用方式(GB/T 7714)：
Qiao Ye,Chaonan Zhang,Zhenlu Wang,et al.Induction of oxidative stress, apoptosis and DNA damage by koumine in Tetrahymena thermophila.[J].PloS one.2019,14(2):e0212231.doi:10.1371/journal.pone.0212231.
APA：
Qiao Ye,Chaonan Zhang,Zhenlu Wang,Yongyong Feng,Aiguo Zhou...&Jixing Zou.(2019).Induction of oxidative stress, apoptosis and DNA damage by koumine in Tetrahymena thermophila..PloS one,14,(2)
MLA：
Qiao Ye,et al."Induction of oxidative stress, apoptosis and DNA damage by koumine in Tetrahymena thermophila.".PloS one 14..2(2019):e0212231
