机构：[1]Department of Otolaryngology, Head and Neck Surgery, Nanfang Hospital,Southern Medical University, 1838 Guangzhou Avenue North, Guangzhou510515, China[2]Department of Otolaryngology, Gaoyao DistrictTraditional Chinese Medicine Hospital of Zhaoqing, No.3 of FuQian Avenue,Zhaoqing 526100, Guangdong, China
Laryngopharyngeal reflux (LPR), with its increasing morbidity, is attracting considerable attention. In recent years, the causal role between LPR and laryngeal carcinoma has been debated. The main harmful component of LPR is pepsin, which has been shown to induce mucosal inflammation by damaging the mucous membrane. Thus, pepsin is linked to an increased risk of laryngeal carcinoma, although the potential mechanism remains largely unknown.
The human laryngeal carcinoma cell lines Hep-2 and Tu212 were exposed to different pepsin concentrations and the morphology, proliferation, migration, secretion of inflammatory cytokines, and epithelial-mesenchymal transition (EMT) of the cells were assessed. To evaluate whether interleukin-8 (IL-8) had a causal relationship with pepsin and EMT, an IL-8 inhibitor was used to suppress IL-8 secretion during pepsin exposure and the expression of EMT markers, cell proliferation, and migration were analyzed.
Pepsin promoted proliferation, colony formation, migration, and IL-8 secretion of Hep-2 and Tu212 cells in vitro. Furthermore, increased pepsin concentrations changed the morphology of Hep-2 and Tu212 cells; levels of the epithelial marker E-cadherin were reduced and those of mesenchymal markers vimentin and β-catenin and the transcription factors snail and slug were elevated. A similar effect was observed in laryngeal carcinoma tissues using immunohistochemistry. IL-8 level was reduced and EMT was restored when pepsin was inhibited by pepstatin. EMT was weakened after exposure to the IL-8 inhibitor, with significant reduction in pepsin-induced cell proliferation and migration.
Pepsin may induce EMT in laryngeal carcinoma through the IL-8 signaling pathway, which indicates that it has potential role in enhancing cell proliferation and metastasis of laryngeal carcinoma.
基金：
The National Natural Science Foundation of
China (No. 81400452).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|3 区医学
小类|3 区肿瘤学
最新[2025]版：
大类|3 区医学
小类|3 区肿瘤学
第一作者：
第一作者机构：[1]Department of Otolaryngology, Head and Neck Surgery, Nanfang Hospital,Southern Medical University, 1838 Guangzhou Avenue North, Guangzhou510515, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Tan Jia-Jie,Wang Lu,Mo Ting-Ting,et al.Pepsin promotes IL-8 signaling-induced epithelial-mesenchymal transition in laryngeal carcinoma.[J].Cancer cell international.2019,19:64.doi:10.1186/s12935-019-0772-7.
APA：
Tan Jia-Jie,Wang Lu,Mo Ting-Ting,Wang Jie,Wang Mei-Gui&Li Xiang-Ping.(2019).Pepsin promotes IL-8 signaling-induced epithelial-mesenchymal transition in laryngeal carcinoma..Cancer cell international,19,
MLA：
Tan Jia-Jie,et al."Pepsin promotes IL-8 signaling-induced epithelial-mesenchymal transition in laryngeal carcinoma.".Cancer cell international 19.(2019):64
