iTRAQ-Based Protein Profiling in CUMS Rats Provides Insights into Hippocampal Ribosome Lesion and Ras Protein Changes Underlying Synaptic Plasticity in Depression.
机构：[1]School of Chinese Medicine, The University of Hong Kong, 999077, Hong Kong[2]School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, 510515, China[3]Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province, 510515, China
Hippocampal atrophy is one of the key changes in the brain implicated in the biology of depression. However, the precise molecular mechanism remains poorly understood due to a lack of biomarkers. In this research, we used behavioral experiments to evaluate anxiety and anhedonia levels in depressed rats using chronic unpredictable mild stress (CUMS) modeling. We also used isobaric tag for relative and absolute quantitation (iTRAQ) to identify the differentially expressed hippocampal proteins between depressed and normal rats. Bioinformatics analyses were also performed for a better understanding. The results showed that CUMS rats had higher anxiety and anhedonia levels than control rats, along with hippocampal lesions. Through iTRAQ and bioinformatics analyses, we found that ribosome proteins were significantly downregulated and Ras proteins exhibited a mixed change in the hippocampus of depressed rats. These findings suggest that the expression of hippocampal ribosome lesions and Ras proteins is significantly different in depressed rats than in control rats, providing new insights into the neurobiology of depression.
基金：
This work was supported by the National Natural Science
Foundation of China (81873359 and 81603474), the Natural
Science Foundation of Guangdong Province (2016A030313522
and 2016A030310383), and the Science and Technology
Program of Guangzhou (201707010041), China.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|3 区医学
小类|3 区神经科学
最新[2025]版：
大类|3 区医学
小类|4 区神经科学
第一作者：
第一作者机构：[1]School of Chinese Medicine, The University of Hong Kong, 999077, Hong Kong
共同第一作者：
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推荐引用方式(GB/T 7714)：
Jialing Zhang,Zhinan Zhang,Jiping Zhang,et al.iTRAQ-Based Protein Profiling in CUMS Rats Provides Insights into Hippocampal Ribosome Lesion and Ras Protein Changes Underlying Synaptic Plasticity in Depression.[J].Neural plasticity.2019,2019:7492306.doi:10.1155/2019/7492306.
APA：
Jialing Zhang,Zhinan Zhang,Jiping Zhang,Zheng Zhong,Zengyu Yao...&Yong Huang.(2019).iTRAQ-Based Protein Profiling in CUMS Rats Provides Insights into Hippocampal Ribosome Lesion and Ras Protein Changes Underlying Synaptic Plasticity in Depression..Neural plasticity,2019,
MLA：
Jialing Zhang,et al."iTRAQ-Based Protein Profiling in CUMS Rats Provides Insights into Hippocampal Ribosome Lesion and Ras Protein Changes Underlying Synaptic Plasticity in Depression.".Neural plasticity 2019.(2019):7492306
