机构：[1]Department of Orthopedic, The Third Affiliated Hospital of Southern Medical University, Orthopaedic Hospital of Guangdong Province, The Third Clinical Medical College of Southern Medical University, Guangzhou, China[2]Department of Orthopedic, Guangdong No. 2 Provincial People's Hospital of Southern Medical University, Guangzhou, China[3]Department of Orthopedic, Traditional Chinese Medicine Hospital of Puning City, Orthopaedic Hospital of Puning City, Puning, China[4]Department of Orthopedic, Guangdong No. 2 Provincial People's Hospital of Southern Medical University, The Third Clinical Medical College of Southern Medical University, Guangzhou, China[5]Department of Spine Surgery, Guangdong Second Provincial General Hospital, 510317, Guangzhou, China
Spinal cord injury (SCI) is lead to locomotor impairment because of neurological damage after following trauma. Quercetin (Que) has been confirmed to have a neuro-protective effect during nerve damage processes. The purpose of this study was to determine the roles of Que in functional recovery, cavity formation, astrocyte activation, and nerve regeneration following SCI. Sprague-Dawley rats were randomly divided into three groups: Sham group, SCI group, and Que + SCI group. A rat model of SCI was made at T10 using the modified Allen's method. In the Que + SCI group, animals underwent laminectomy and were then intraperitoneally injected with 20 mg/kg Que for 7 days. Locomotor function was determined with the Basso, Beattie, Bresnahan (BBB) scores at 1, 3, 5, and 7 days post-injury. At 7 days post-injury, somatosensory evoked potentials (SEPs) and motor evoked potentials (MEPs) were recorded. Hematoxylin-Eosin (HE) staining was used to investigate cavity formation. Astrocyte activation was assayed by immunohistochemistry staining with an antibody specific for glial fibrillary acidic protein (GFAP), as well as the expression of GFAP and S100β. Axons were stained using an antibody specific for neurofilament 200 (NF200) and 5-hydroxytryptamine (5-HT). In addition, the protein level of BDNF, p-JNK2, and p-STAT3 was detected using Western blot. Que promoted locomotor function and electrophysiological recovery, reduced cavity formation, contributed to astrocyte activation and axonal regeneration after acute SCI. Moreover, Que up-regulated the expression of BDNF, but reduced p-JNK2 and p-STAT3 expression after acute SCI. Taken together, Que promoted locomotor and electrophysiological functional recovery, astrocyte activation and axonal regeneration after acute SCI, possibly through BDNF and JAK2/STAT3 signaling pathways.
基金：
Funding information
Medical Science Research Foundation of
Guangdong Province, Grant number:
A2012138; The Science and Technology
Plan Foundation of Guangdong Province,
Grant number: 2012B031800266
第一作者机构：[1]Department of Orthopedic, The Third Affiliated Hospital of Southern Medical University, Orthopaedic Hospital of Guangdong Province, The Third Clinical Medical College of Southern Medical University, Guangzhou, China[5]Department of Spine Surgery, Guangdong Second Provincial General Hospital, 510317, Guangzhou, China
通讯作者：
通讯机构：[1]Department of Orthopedic, The Third Affiliated Hospital of Southern Medical University, Orthopaedic Hospital of Guangdong Province, The Third Clinical Medical College of Southern Medical University, Guangzhou, China[*1]Department of Orthopedic, The Third Affiliated Hospital of Southern Medical University, Orthopaedic Hospital of Guangdong Province, The Third Clinical Medical College of Southern Medical University, No. 183 Zhongshan Road West, Tianhe District, 510630 Guangzhou, China
推荐引用方式(GB/T 7714)：
Wang Yeyang,Li Wenjun,Wang Mingsen,et al.Quercetin reduces neural tissue damage and promotes astrocyte activation after spinal cord injury in rats.[J].JOURNAL OF CELLULAR BIOCHEMISTRY.2018,119(2):2298-2306.doi:10.1002/jcb.26392.
APA：
Wang Yeyang,Li Wenjun,Wang Mingsen,Lin Chuangxin,Li Guitao...&Jin Dadi.(2018).Quercetin reduces neural tissue damage and promotes astrocyte activation after spinal cord injury in rats..JOURNAL OF CELLULAR BIOCHEMISTRY,119,(2)
MLA：
Wang Yeyang,et al."Quercetin reduces neural tissue damage and promotes astrocyte activation after spinal cord injury in rats.".JOURNAL OF CELLULAR BIOCHEMISTRY 119..2(2018):2298-2306
