机构：[1]Clinical Laboratory of Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515[2]Department of Nephrology, The Traditional Chinese Medicine Hospital, Affiliated to Southwest Medical University, Luzhou,Sichuan 646000[3]South China Institute of Microbial Ecology and Health[4]Department of Rheumatology, Nanfang Hospital[5]Guangdong Key Laboratory of Biochip Technology, Southern Medical University,Guangzhou, Guangdong 510515, P.R. China
Systemic lupus erythematosus (SLE) is a common autoimmune disease, which features the secretion of antibodies directed against autoantigens in vivo. In the present study, a peptide microarray was developed to detect the epitopes recognized by autoantibodies in patients with SLE for an effective method of diagnosis. SLE‑associated epitopes in 14 autoantigens were predicted using the antigenic epitope prediction software DNA star. Peptides were synthesized based on the predicted antigenic epitopes and immobilized on a slide surface and developed into a peptide microarray. Using this peptide microarray the autoantibodies in 120 patients with SLE and 110 healthy subjects were detected. A total of 73 potential antigenic epitopes in 14 autoantigens were predicted and screened. The peptide microarray based on the 73 epitopes was used to detect the autoantibodies in patients with SLE. A total of 14 epitopes with potential diagnostic values were screened out. The sensitivity and specificity of the 14 epitopes for the diagnosis of SLE were 71.6 and 85.8%, respectively. An optimal set of epitopes for SLE diagnosis was obtained. As individual patients had a specific autoantibody spectrum it was possible to detect autoantibodies in SLE and perform the diagnosis of SLE using the peptide microarray.
基金：
the National Natural Science Foundation of China (grant no. 320.6750.16039) and President Foundation of Nanfang Hospital, Southern Medical University (grant no. 2017C033)
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|4 区医学
小类|4 区医学：研究与实验4 区肿瘤学
最新[2025]版：
大类|4 区医学
小类|4 区医学：研究与实验4 区肿瘤学
第一作者：
第一作者机构：[1]Clinical Laboratory of Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515
共同第一作者：
通讯作者：
通讯机构：[1]Clinical Laboratory of Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515[2]Department of Nephrology, The Traditional Chinese Medicine Hospital, Affiliated to Southwest Medical University, Luzhou,Sichuan 646000[5]Guangdong Key Laboratory of Biochip Technology, Southern Medical University,Guangzhou, Guangdong 510515, P.R. China[*1]Clinical Laboratory of Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, Guangzhou, Guangdong 510515, P.R. China[*2]Department of Nephrology, The Traditional Chinese Medicine Hospital, Affiliated to Southwest Medical University, 182 Chunhui Road, Luzhou, Sichuan 646000, P.R. China
推荐引用方式(GB/T 7714)：
Li Xin,Li Haixia,Hu Qiongdan,et al.Detection of epitopes in systemic lupus erythematosus using peptide microarray.[J].Molecular medicine reports.2018,17(5):6533-6541.doi:10.3892/mmr.2018.8710.
APA：
Li Xin,Li Haixia,Hu Qiongdan,Lin Jinfei,Zhang Qiong...&Qiu Yurong.(2018).Detection of epitopes in systemic lupus erythematosus using peptide microarray..Molecular medicine reports,17,(5)
MLA：
Li Xin,et al."Detection of epitopes in systemic lupus erythematosus using peptide microarray.".Molecular medicine reports 17..5(2018):6533-6541
