机构:[1]Guizhou Provincial Key Laboratory for Regenerative Medicine, Tissue Engineering and Stem Cell Research Center, Department of Immunology, School of Basic Medicine, Guizhou Medical University, Guiyang, China[2]Affiliated Hospital of Guizhou Medical University, State Key Laboratory for Medicinal Plant Efficacy and Utilization, Guizhou Medical University, Guiyang, China[3]Second Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, China广东省中医院[4]School of Food Science and Engineering, Foshan University, Foshan, Guangdong Province, China[5]Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China[6]Guizhou Provincial Key Laboratory for Regenerative Medicine, Tissue Engineering and Stem Cell Research Center, Guizhou Medical University, Guiyang, China
Vanillin (4-hydroxy-3-methoxybenzaldehyde), a food additive with rich milk flavor, is commonly used in the food, beverage and cosmetic industries. However, excessive consumption of vanillin may cause liver and kidney damage. Therefore, methods for detecting and controlling the level of vanillin in food, especially in infant powder, have important practical significance. In this study, we established a colorimetric assay for vanillin detection. The detection was performed under high-temperature and acidic conditions, which can induce the reaction of the aldehyde group of vanillin with the amino group of o-toluidine. The resulting product had a maximum absorption at 363 nm, which was quantified by a UV spectrophotometer. This assay had a limit of detection (LOD) of 1 pg mL-1 and a linear range between 1 μg mL-1 and 100 μg mL-1. The average recoveries at three spiked levels were in the range from 91.1% to 101.6% with a relative standard deviation (RSD) of 4.62% ~ 7.27%.
基金:
Key
Laboratory of Regenerative Biology, Guangzhou
Institutes of Biomedicine and Health, Chinese
Academy of Sciences, Stem Cell and Molecular
Diagnostic Laboratory. This study was also
supported by the Guangdong Science and
Technology Foundation (2014B020212011).
第一作者机构:[1]Guizhou Provincial Key Laboratory for Regenerative Medicine, Tissue Engineering and Stem Cell Research Center, Department of Immunology, School of Basic Medicine, Guizhou Medical University, Guiyang, China[2]Affiliated Hospital of Guizhou Medical University, State Key Laboratory for Medicinal Plant Efficacy and Utilization, Guizhou Medical University, Guiyang, China[6]Guizhou Provincial Key Laboratory for Regenerative Medicine, Tissue Engineering and Stem Cell Research Center, Guizhou Medical University, Guiyang, China
共同第一作者:
通讯作者:
通讯机构:[1]Guizhou Provincial Key Laboratory for Regenerative Medicine, Tissue Engineering and Stem Cell Research Center, Department of Immunology, School of Basic Medicine, Guizhou Medical University, Guiyang, China[2]Affiliated Hospital of Guizhou Medical University, State Key Laboratory for Medicinal Plant Efficacy and Utilization, Guizhou Medical University, Guiyang, China[4]School of Food Science and Engineering, Foshan University, Foshan, Guangdong Province, China[5]Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
推荐引用方式(GB/T 7714):
Zhao Jin,Xia Haixiong,Yu Tingyu,et al.A colorimetric assay for vanillin detection by determination of the luminescence of o-toluidine condensates.[J].PLOS ONE.2018,13(4):doi:10.1371/journal.pone.0194010.
APA:
Zhao Jin,Xia Haixiong,Yu Tingyu,Jin Lu,Li Xuehua...&He Zhixu.(2018).A colorimetric assay for vanillin detection by determination of the luminescence of o-toluidine condensates..PLOS ONE,13,(4)
MLA:
Zhao Jin,et al."A colorimetric assay for vanillin detection by determination of the luminescence of o-toluidine condensates.".PLOS ONE 13..4(2018)