Identification of Key Candidate Proteins and Pathways Associated with Temozolomide Resistance in Glioblastoma Based on Subcellular Proteomics and Bioinformatical Analysis.
机构：[1]Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China[2]The Laboratory for Precision Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China[3]Department of Neurosurgery,The First Affiliated Hospital, Southwest Medical University, Luzhou, Sichuan 646000, China[4]Department of General Intensive Care Unit,TheThird Affiliated Hospital of Sun Yat-sen University of Medical Sciences, Guangzhou 510630, China中山大学附属第三医院[5]Guangdong Provincial Key Laboratory of Molecular Oncologic Pathology, Guangzhou, Guangdong 510515, China[6]School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China[7]Center for Clinical Medical Education, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China[8]Nanfang Glioma Center, Guangzhou, Guangdong 510515, China
TMZ resistance remains one of the main reasons why treatment of glioblastoma (GBM) fails. In order to investigate the underlying proteins and pathways associated with TMZ resistance, we conducted a cytoplasmic proteome research of U87 cells treated with TMZ for 1 week, followed by differentially expressed proteins (DEPs) screening, KEGG pathway analysis, protein-protein interaction (PPI) network construction, and validation of key candidate proteins in TCGA dataset. A total of 161 DEPs including 65 upregulated proteins and 96 downregulated proteins were identified. Upregulated DEPs were mainly related to regulation in actin cytoskeleton, focal adhesion, and phagosome and PI3K-AKT signaling pathways which were consistent with our previous studies. Further, the most significant module consisted of 28 downregulated proteins that were filtered from the PPI network, and 9 proteins (DHX9, HNRNPR, RPL3, HNRNPA3, SF1, DDX5, EIF5B, BTF3, and RPL8) among them were identified as the key candidate proteins, which were significantly associated with prognosis of GBM patients and mainly involved in ribosome and spliceosome pathway. Taking the above into consideration, we firstly identified candidate proteins and pathways associated with TMZ resistance in GBM using proteomics and bioinformatic analysis, and these proteins could be potential biomarkers for prevention or prediction of TMZ resistance in the future.
基金：
National Natural Science
Foundation of China (Grant nos. 81773290, 81472315, and
81302229), Natural Science Foundation of Guangdong
Province (2014A030313167 and 2017A030313497), National
Key Technology Research and Development Program
of the Ministry of Science and Technology of China
(2014BAI04B01), and President Foundation of Nanfang
Hospital, Southern Medical University (2017C025).
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|3 区生物
小类|3 区生物工程与应用微生物4 区医学：研究与实验
最新[2025]版：
大类|4 区医学
小类|4 区生物工程与应用微生物4 区医学：研究与实验
JCR分区：
出版当年[2016]版：
Q2BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
第一作者机构：[1]Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China[2]The Laboratory for Precision Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China[2]The Laboratory for Precision Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China[5]Guangdong Provincial Key Laboratory of Molecular Oncologic Pathology, Guangzhou, Guangdong 510515, China[8]Nanfang Glioma Center, Guangzhou, Guangdong 510515, China
推荐引用方式(GB/T 7714)：
Yi Guo-Zhong,Xiang Wei,Feng Wen-Yan,et al.Identification of Key Candidate Proteins and Pathways Associated with Temozolomide Resistance in Glioblastoma Based on Subcellular Proteomics and Bioinformatical Analysis.[J].BIOMED RESEARCH INTERNATIONAL.2018,2018:doi:10.1155/2018/5238760.
APA：
Yi Guo-Zhong,Xiang Wei,Feng Wen-Yan,Chen Zi-Yang,Li Yao-Min...&Liu Ya-Wei.(2018).Identification of Key Candidate Proteins and Pathways Associated with Temozolomide Resistance in Glioblastoma Based on Subcellular Proteomics and Bioinformatical Analysis..BIOMED RESEARCH INTERNATIONAL,2018,
MLA：
Yi Guo-Zhong,et al."Identification of Key Candidate Proteins and Pathways Associated with Temozolomide Resistance in Glioblastoma Based on Subcellular Proteomics and Bioinformatical Analysis.".BIOMED RESEARCH INTERNATIONAL 2018.(2018)
