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Linc-ROR Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Functioning as a Competing Endogenous RNA for miR-138 and miR-145.

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资源类型：
Pubmed体系：
作者：
Lu Feng[1] * ; Liu Shi[1] * ; Ying-fei Lu[2] ; Bin Wang[1] ; Tao Tang[3] ; Wei-ming Fu[4] ; Wei He[5] ; Gang Li[1,*2] ; Jin-fang Zhang[5,6,*1] ;
机构： [1]Department of Orthopaedics & Traumatology, Li Ka Shing Institute of Health Sciences and Lui Che Woo Institute of Innovative Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong SAR, China [2]Central Laboratory, The Affiliated Jiangning Hospital with Nanjing Medical University, Nanjing, Jiangsu 211100, China [3]Department of Obstetrics & Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China [4]Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China [5]Key Laboratory of Orthopaedics and Traumatology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, The First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, China [6]Laboratory of Orthopaedics & Traumatology, Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou, China
出处：
DOI： 10.1016/j.omtn.2018.03.004
ISSN：

摘要：
Long noncoding RNAs (lncRNAs), which serve as important and powerful regulators of various biological activities, have gained widespread attention in recent years. Emerging evidence has shown that some lncRNAs play important regulatory roles in osteoblast differentiation of mesenchymal stem cells (MSCs), suggesting a potential therapeutic strategy for bone fracture. As a recently identified lncRNA, linc-ROR was reported to mediate the reprogramming ability of differentiated cells into induced pluripotent stem cells (iPSCs) and human embryonic stem cells (ESCs) self-renewal. However, other functions of linc-ROR remain elusive. In this study, linc-ROR was found to be upregulated during osteogenesis of human bone-marrow-derived MSCs. Ectopic expression of linc-ROR significantly accelerated, whereas knockdown of linc-ROR suppressed, osteoblast differentiation. Using bioinformatic prediction and luciferase reporter assays, we demonstrated that linc-ROR functioned as a microRNA (miRNA) sponge for miR-138 and miR-145, both of which were negative regulators of osteogenesis. Further investigations revealed that linc-ROR antagonized the functions of these two miRNAs and led to the de-repression of their shared target ZEB2, which eventually activated Wnt/β-catenin pathway and hence potentiated osteogenesis. Taken together, linc-ROR modulated osteoblast differentiation by acting as a competing endogenous RNA (ceRNA), which may shed light on the functional characterization of lncRNAs in coordinating osteogenesis. Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

基金：
This work was supported by grants from the National Natural Science Foundation of China (81772404, 81773066, 81371946, 81430049, and 81772322) and the Hong Kong Government Research Grant Council, General Research Fund (14119115, 14160917, 9054014 N_CityU102/ 15, and T13-402/17-N). This study was also supported in part by the SMART program, the Lui Che Woo Institute of Innovative Medicine, and The Chinese University of Hong Kong, and research was made possible by resources donated by Lui Che Woo Foundation Limited. We thank Prof. Houqi Liu for kindly providing the sh-linc-ROR plasmid.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类 | 2 区 医学
小类 | 2 区 医学：研究与实验
最新[2025]版：
大类 | 2 区 医学
小类 | 2 区 医学：研究与实验
第一作者：
第一作者机构： [1]Department of Orthopaedics & Traumatology, Li Ka Shing Institute of Health Sciences and Lui Che Woo Institute of Innovative Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong SAR, China
共同第一作者：
通讯作者：
通讯机构： [1]Department of Orthopaedics & Traumatology, Li Ka Shing Institute of Health Sciences and Lui Che Woo Institute of Innovative Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong SAR, China [5]Key Laboratory of Orthopaedics and Traumatology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, The First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, China [6]Laboratory of Orthopaedics & Traumatology, Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou, China [*1]The First Affiliated Hospital of Guangzhou University of Chinese Medicine, The First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, China. [*2]DPhil (Oxon), Room 904, Department of Orthopaedics & Traumatology, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, NT, Hong Kong, SAR, China.
推荐引用方式(GB/T 7714)：
Lu Feng,Liu Shi,Ying-fei Lu,et al.Linc-ROR Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Functioning as a Competing Endogenous RNA for miR-138 and miR-145.[J].Molecular therapy. Nucleic acids.2018,11:345-353.doi:10.1016/j.omtn.2018.03.004.
APA：
Lu Feng,Liu Shi,Ying-fei Lu,Bin Wang,Tao Tang...&Jin-fang Zhang.(2018).Linc-ROR Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Functioning as a Competing Endogenous RNA for miR-138 and miR-145..Molecular therapy. Nucleic acids,11,
MLA：
Lu Feng,et al."Linc-ROR Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Functioning as a Competing Endogenous RNA for miR-138 and miR-145.".Molecular therapy. Nucleic acids 11.(2018):345-353

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