机构：[1]Department of Traditional Chinese Medicine, The Third Affiliated Hospitalof Sun Yet-sen University, Guangzhou, Guangdong 510000, P.R. China中山大学附属第三医院
This study aimed to investigate the expression of microRNA (miRNA) 299 and miRNA-7706 in patients with hepatocellular carcinoma (HCC), and to explore their effects on proliferation of SK-HEP-1 HCC cells. Expression of miRNA-299 and miRNA-7706 in tumor tissue (HCC group) and adjacent healthy tissue (>30 mm away from the tumor tissue) of 179 patients with HCC was determined by real-time polymerase chain reaction (qRT-PCR). miR-299 mimics and miR-7706 mimics were transfected into SK-HEP-1 HCC cells by RNA transfection. The proliferation and invasion of SK-HEP-1 cells were detected by CCK-8 kit and Transwell kit, respectively. Compared with adjacent tissues, expression levels of miRNA-299 and miRNA-7706 in HCC group were significantly downregulated. Analyses on the correlation between the expression of miRNA-299 and miRNA-7706 and clinical factors showed that expression levels of miRNA-299 and miRNA-7706 were significantly correlated with pathological stages and lymph node metastasis. ROC curve analysis showed that the areas under the curve were 0.837 and 0.845 for miRNA-299 and miRNA-7706 in the prediction of HCC, respectively. Survival analysis showed that the 5-year overall survival rate of patients with high expression levels of miRNA-299 and miRNA-7706 was significantly different from that of patients with low expression levels (P=0.016). Compared with cells transfected with scramble mimics, proliferation and invasion abilities of SK-HEP-1 cells transfected with miR-299 mimics and miRNA-7706 were significantly weakened. Results suggested that downregulation of miRNA-299 and miRNA-7706 can inhibit the proliferation of HCC cells and can be used as a new target for the treatment of HCC.
基金：
Special Scientific Expenditure for Business Construction of State TCM Clinical Research Base, State Administration of TCM of the People's Republic of China. State TCM Scientific Task [2016] 20, no. JDZX2015173.
第一作者机构：[1]Department of Traditional Chinese Medicine, The Third Affiliated Hospitalof Sun Yet-sen University, Guangzhou, Guangdong 510000, P.R. China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Traditional Chinese Medicine, The Third Affiliated Hospitalof Sun Yet-sen University, Guangzhou, Guangdong 510000, P.R. China[*1]Department of Traditional Chinese Medicine, The Third Affiliated Hospital of Sun Yet-sen University, 600 Tianhe Road, Guangzhou, Guangdong 510000, P.R. China
推荐引用方式(GB/T 7714)：
Wang Fenglin,Dai Min,Chen Hongjie,et al.Prognostic value of hsa-mir-299 and hsa-mir-7706 in hepatocellular carcinoma.[J].ONCOLOGY LETTERS.2018,16(1):815-820.doi:10.3892/ol.2018.8710.
APA：
Wang Fenglin,Dai Min,Chen Hongjie,Li Yue,Zhang Jiongshan...&Yang Hongzhi.(2018).Prognostic value of hsa-mir-299 and hsa-mir-7706 in hepatocellular carcinoma..ONCOLOGY LETTERS,16,(1)
MLA：
Wang Fenglin,et al."Prognostic value of hsa-mir-299 and hsa-mir-7706 in hepatocellular carcinoma.".ONCOLOGY LETTERS 16..1(2018):815-820
