Chaihu-Shugan-San Decoction Modulates Intestinal Microbe Dysbiosis and Alleviates Chronic Metabolic Inflammation in NAFLD Rats via the NLRP3 Inflammasome Pathway.
机构：[1]School of Nursing, Jinan University, No. 601 Huangpu AvenueWest, Guangzhou, Guangdong 510632, China [2]School of Traditional Chinese Medicine, Jinan University, No. 601 Huangpu AvenueWest, Guangzhou, Guangdong 510632, China [3]Department of Pathology, School of Basic Medicine, Jinan University, No. 601 Huangpu AvenueWest, Guangzhou, Guangdong 510632, China [4]Department of Traditional Chinese Medicine, First Affiliated Hospital, Jinan University, No. 613 Huangpu AvenueWest, Guangzhou, Guangdong 510632, China
We evaluate the effects of the Chaihu-Shugan-San decoction on intestinal microbe dysbiosis and chronic metabolic inflammation via the NLRP3 pathway in NAFLD rats that were fed a high-fat diet. Twenty-four SD rats (male, six weeks old, 200 ± 20 g) were randomly divided into three groups: normal control group (NC group), high-fat diet-fed group (HFD group), and Chaihu-Shugan-San decoction intervention group (CH group). The NC group rats were given standard feed, the HFD group rats were all fed a high-fat diet (83% standard feed + 10% lard oil + 5% sucrose + 1.5% cholesterol + 0.5% cholate), and the CH group rats were given a HFD plus Chaihu-Shugan-San at 9.6 g•kg-1•d-1. Body composition, serum and liver lipids, inflammatory markers, intestinal microbial population, and the NLRP3 pathway-associated protein were assessed. The results showed that Chaihu-Shugan-San decoction significantly reduced body weight and total fat mass and the levels of serum LPS, TG, TNF-α, IL-1β, and IL-18, as well as liver TC, TG, TNF-α, IL-1β, and IL-18 (P < 0.05). The abundance of Enterobacteriaceae (0.375% versus 0.064%, P < 0.05), Staphylococcaceae families (0.049% versus 0.016%, P < 0.05) and Veillonella genus (0.096% versus 0.009%, P < 0.01) significantly decreased, whereas the abundance of Anaeroplasma genus (0.0005% versus 0.0178%, P < 0.01) significantly increased. The expression levels of NLRP3, ASC, and Caspase-1 were changed significantly (P < 0.05). In summary, the Chaihu-Shugan-San decoction modulated intestinal microbe dysbiosis, reduced fat accumulation, and alleviated inflammatory factor expression, which are all processes related to the NLRP3 inflammasome pathway in NAFLD rats.
基金：
NationalNatural Science Foundation of China (nos. 81774165
and 81573844), Natural Science Foundation of Guangdong in
China (no. 2016A030313824), Traditional Chinese Medicine
Bureau of Guangdong in China (no. 20161065), and National
Health and Family Planning Commission of Guangdong in
China (no. A2016583, A2017228, A2017140).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|4 区医学
小类|3 区全科医学与补充医学
最新[2025]版：
无
第一作者：
第一作者机构：[1]School of Nursing, Jinan University, No. 601 Huangpu AvenueWest, Guangzhou, Guangdong 510632, China [2]School of Traditional Chinese Medicine, Jinan University, No. 601 Huangpu AvenueWest, Guangzhou, Guangdong 510632, China
通讯作者：
推荐引用方式(GB/T 7714)：
Liang Yinji,Zhang Yupei,Deng Yuanjun,et al.Chaihu-Shugan-San Decoction Modulates Intestinal Microbe Dysbiosis and Alleviates Chronic Metabolic Inflammation in NAFLD Rats via the NLRP3 Inflammasome Pathway.[J].Evidence-based complementary and alternative medicine : eCAM.2018,2018:9390786.doi:10.1155/2018/9390786.
APA：
Liang Yinji,Zhang Yupei,Deng Yuanjun,Liang Shu,He Yifang...&Yang Qinhe.(2018).Chaihu-Shugan-San Decoction Modulates Intestinal Microbe Dysbiosis and Alleviates Chronic Metabolic Inflammation in NAFLD Rats via the NLRP3 Inflammasome Pathway..Evidence-based complementary and alternative medicine : eCAM,2018,
MLA：
Liang Yinji,et al."Chaihu-Shugan-San Decoction Modulates Intestinal Microbe Dysbiosis and Alleviates Chronic Metabolic Inflammation in NAFLD Rats via the NLRP3 Inflammasome Pathway.".Evidence-based complementary and alternative medicine : eCAM 2018.(2018):9390786
