机构：[1]Research Center of Translational Medicine, the First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong Province, China中山大学附属第一医院[2]Department of Pathology, Sun Yat-Sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative innovation Center for Cancer Medicine, Guangzhou, China[3]Department of Physiology, Basic Medical College, Guangzhou University of Chinese Medicine, University Town, Guangzhou, China[4]School of Chinese Pharmaceutical Science, Guangzhou University of Chinese Medicine, University Town, Guangzhou, China深圳市中医院深圳医学信息中心
17β-estradiol (E2) has been shown to have beneficial effects on the cardiovascular system. We previously demonstrated that E2 increases striatin levels and inhibits migration in vascular smooth muscle cells. The objective of the present study was to investigate the effects of E2 on the regulation of striatin expression in human umbilical vein endothelial cells (HUVECs). We demonstrated that E2 increased striatin protein expression in a dose- and time-dependent manner in HUVECs. Pretreatment with ICI 182780 or the phosphatidylinositol-3 kinase inhibitor, wortmannin, abolished E2-mediated upregulation of striatin protein expression. Treatment with E2 resulted in Akt phosphorylation in a time-dependent manner. Moreover, silencing striatin significantly inhibited HUVEC migration, while striatin overexpression significantly promoted HUVEC migration. Finally, E2 enhanced HUVEC migration, which was inhibited by silencing striatin. In conclusion, our results demonstrated that E2-mediated upregulation of striatin promotes cell migration in HUVECs.
基金：
the National
Natural Science Foundation of China (Grant No.
81774107 to J.W.X.), Department of Education of
Guangdong Province (Grant No. yq2014045 to J.
W.X.), Guangzhou University of Chinese Medicine
(Grant No. QNYC20170101 to J.W.X.), by the
Guangdong Natural Science Foundation (Grant No.
2014A030310059 to S.Z. and Grant No.
2014A030313105 to Y.X.)
第一作者机构：[1]Research Center of Translational Medicine, the First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong Province, China
通讯作者：
推荐引用方式(GB/T 7714)：
Zheng Shuhui,Sun Peng,Liu Haimei,et al.17β-estradiol upregulates striatin protein levels via Akt pathway in human umbilical vein endothelial cells.[J].PLOS ONE.2018,13(8):doi:10.1371/journal.pone.0202500.
APA：
Zheng Shuhui,Sun Peng,Liu Haimei,Li Runmei,Long Lingli...&Xu Jinwen.(2018).17β-estradiol upregulates striatin protein levels via Akt pathway in human umbilical vein endothelial cells..PLOS ONE,13,(8)
MLA：
Zheng Shuhui,et al."17β-estradiol upregulates striatin protein levels via Akt pathway in human umbilical vein endothelial cells.".PLOS ONE 13..8(2018)
