机构：[1]Department of Pathophysiology, Institute of Brain Science Research, Key Laboratory of State Administration of Traditional Chinese Medicine of the People’s Republic of China, School of Medicine, Jinan University, Guangzhou, Guangdong 510632, China [2]Department of Pathology, Guangzhou Overseas Chinese Hospital, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, China
Neural stem cells (NSCs) hold great potential for the treatment of Alzheimer's disease (AD) through both cellular replacement and their secretion of trophic factors. Lycopene is a potent β-carotenoid antioxidant that has been shown to ameliorate oxidative damage in previous studies. However, it is unclear if lycopene can interact with NSCs to induce the secretion of growth factors, and whether pretreatment with lycopene will allow NSCs to secrete enough trophic factors to reduce oxidative damage to neurons. We pretreated cultured NSCs with lycopene, then applied the lycopene-treated-NSC-conditioned media (Ly-NSC-CM) to primary neuronal cultures exposed to tert-butyl hydroperoxide (t-BHP) to induce oxidative damage. We found that lycopene promoted the secretion of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and vascular endothelial growth factor (VEGF) from NSCs. In addition, Ly-NSC-CM attenuated oxidative stress and reduced t-BHP-induced cell apoptosis. We found an antiapoptotic effect related to inhibited expression of Bax/Bcl-2, cytochrome C, and cleaved caspase-3. Moreover, Ly-NSC-CM increased the levels of synaptic proteins, including synaptophysin (SYP) and postsynaptic density 95 (PSD-95), and activated the PI3K/Akt pathway in cultured neurons. Collectively, these data indicate that Ly-NSC-CM could protect neurons from t-BHP-induced oxidative damage.
基金：
The authors would like to thank the staff of the Department
of Pathophysiology, Institute of Brain Science Research in
Jinan University (Guangzhou, Guangdong Province, China)
for their technical assistance. This work was supported by
grants from the National Natural Science Foundation of
China (nos. 81471236 and 81371442), the training program
for Outstanding Young Teachers in Higher Education Institutions
of Guangdong Province (no. YQ2015024), and the
major special foundation for Science and Technology Planning
Project of Guangzhou City (no. 201506010095).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|2 区生物
小类|3 区细胞生物学
最新[2025]版：
无
第一作者：
第一作者机构：[1]Department of Pathophysiology, Institute of Brain Science Research, Key Laboratory of State Administration of Traditional Chinese Medicine of the People’s Republic of China, School of Medicine, Jinan University, Guangzhou, Guangdong 510632, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Huang Cuiqin,Gan Danhui,Fan Chongzhu,et al.The Secretion from Neural Stem Cells Pretreated with Lycopene Protects against tert-Butyl Hydroperoxide-Induced Neuron Oxidative Damage.[J].Oxidative medicine and cellular longevity.2018,2018:5490218.doi:10.1155/2018/5490218.
APA：
Huang Cuiqin,Gan Danhui,Fan Chongzhu,Wen Caiyan,Li An...&Lu Daxiang.(2018).The Secretion from Neural Stem Cells Pretreated with Lycopene Protects against tert-Butyl Hydroperoxide-Induced Neuron Oxidative Damage..Oxidative medicine and cellular longevity,2018,
MLA：
Huang Cuiqin,et al."The Secretion from Neural Stem Cells Pretreated with Lycopene Protects against tert-Butyl Hydroperoxide-Induced Neuron Oxidative Damage.".Oxidative medicine and cellular longevity 2018.(2018):5490218
