机构：[1]Department of Respiratory and Critical Care Medicine, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory Health, Guangzhou 510120, China.[2]Department of Respiratory, The affiliated hospital of Guangdong medical University, Zhanjiang 524023, China.[3]The Second Affiliated Hospital of Guangdong University of Chinese Medicine, Guangzhou 510120, China.广东省中医院[4]College of Veterinary Medicine, South China Agricultural University, No.483 Wushan Rd, Guangzhou 510642, People’s Republic of China.[5]Department of Biochemistry, Guangdong Medical University, Zhanjiang 524023, China.
To investigate the changes of polyunsaturated fatty acids (PUFAs) and their downstream eicosanoids in patients with asthma, the levels of erythrocyte membrane lipids and plasma lipid metabolites were examined.
Erythrocyte membrane lipids were extracted and esterificated, and then fatty acid compositions were determined by gas chromatography. The concentrations of six eicosanoids of PGE2, TXA2, LTB4, PGE1, 6-k-PGF1α and PGF2α in plasma were measured by ELISA.
The results showed that the contents of erythrocyte membrane fatty acids in patients with asthma were mainly composed of C16:0, C18:0, C18:1, C18:2(n-6), and C20:4(n-6). The ratio n-6/n-3 PUFAs in patients and health persons were (4.42 ± 1.33):1 and (3.21 ± 0.79):1 (p < 0.01), showing statistically significant differences. ELISA results showed that the levels of plasma PGE2, TXB2, and PGE1 in patients were higher than health persons; and the levels of eicosanoids of PGF2α and 6-k-PGF1α were significantly lower in patient group than healthy group (p < 0.05), but LTB4 was no obvious difference (p = 0.09). Increased ratio of n-6/n-3 PUFAs is consistent to the increased levels of pro-inflammatory PGE2 and TXB2 and anti-inflammatory PGE1 originated from C20:4(n-6) and C18:2(n-6), indicating that increased ratio of n-6/n-3 PUFAs and eicosanoids from n-6 PUFAs might promote the progress of airway inflammation of asthma.
Changes of erythrocyte fatty acids, n-6/n-3 PUFAs ratio and the levels of plasma PGE2, TXB2, and PGE1 in patients with asthma were relevant to airway inflammation in some extent. Therefore, it could be proposed that increase of n-3/n-6 PUFAs ratio by diet supplementation of n-3 PUFAs might effectively improve airway inflammation in asthma.
基金：
This study was financially supported by medical health and technology
project of Guangzhou (Grant No. 20161A011061), National natural science
foundation of China (No. 81770079) and Guangdong provincial science and
technology department (No. 2016A020215136).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|3 区生物
小类|4 区生化与分子生物学4 区营养学
最新[2025]版：
大类|2 区医学
小类|2 区生化与分子生物学2 区营养学
第一作者：
第一作者机构：[1]Department of Respiratory and Critical Care Medicine, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory Health, Guangzhou 510120, China.
通讯作者：
通讯机构：[4]College of Veterinary Medicine, South China Agricultural University, No.483 Wushan Rd, Guangzhou 510642, People’s Republic of China.[5]Department of Biochemistry, Guangdong Medical University, Zhanjiang 524023, China.
推荐引用方式(GB/T 7714)：
Jing Zhou,Lifang Chen,Zhenjie Liu,et al.Changes in erythrocyte polyunsaturated fatty acids and plasma eicosanoids level in patients with asthma.[J].Lipids in health and disease.2018,17(1):206.doi:10.1186/s12944-018-0853-y.
APA：
Jing Zhou,Lifang Chen,Zhenjie Liu,Ling Sang,Yimin Li&Dongjuan Yuan.(2018).Changes in erythrocyte polyunsaturated fatty acids and plasma eicosanoids level in patients with asthma..Lipids in health and disease,17,(1)
MLA：
Jing Zhou,et al."Changes in erythrocyte polyunsaturated fatty acids and plasma eicosanoids level in patients with asthma.".Lipids in health and disease 17..1(2018):206
