机构：[1]Department of Rheumatology, Shanghai Guanghua Hospital of Integrated Traditional and Western Medicine, Shanghai, China,[2]Arthritis Institute of Integrated Traditional and Western Medicine, Shanghai Chinese Medicine Research Institute, Shanghai, China,[3]Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan Scientific Research Center, Guangdong Medical University, Dongguan, China,[4]University of Texas Medical School at Houston, Houston, TX, USA,[5]Department of Immunology and Microbiology, Shanghai JiaoTong University School of Medicine, Shanghai, China
Rheumatoid arthritis (RA) is an autoimmune disease that causes chronic inflammation of the joints. Recent evidence indicated the epigenetic changes may contribute to the pathogenesis of RA.
To understand the extent and nature of dysregulated DNA methylation in RA CD4T cells, we performed a genome-wide DNA methylation study in CD4 + T cells in 12 RA patients compared to 12 matched normal healthy controls. Cytosine methylation status was quantified with Illumina methylation 450K microarray.
The DNA methylation profiling showed 383 hyper- and 785 hypo-methylated genes in the CD4 + T cells of the RA patients (p < 3.4 × 10-7). Gene ontology analysis indicated transcript alternative splicing and protein modification mediated by DNA methylation might play an important role in the pathogenesis of RA. In addition, the result showed that human leukocyte antigen (HLA) region including HLA-DRB6, HLA-DQA1 and HLA-E was frequently hypomethylated, but HLA-DQB1 hypermethylated in CpG island region and hypomethylated in CpG shelf region in RA patients. Outside the MHC region, HDAC4, NXN, TBCD and TMEM61 were the most hypermethylated genes, while ITIH3, TCN2, PRDM16, SLC1A5 and GALNT9 are the most hypomethylated genes.
Genome-wide DNA methylation profile revealed significant DNA methylation change in CD4 + T cells from patients with RA.
基金：
This work was funded by the National Natural Science Funds of
China (81273979), Shanghai clinical base construction of traditional
Chinese medicine (ZY3-LCPT-1), Shanghai intensive
entity construction of integrated traditional and western medicine
rheumatoid arthritis (zxbz2012-05) and Shanghai clinical intensive
subject construction of traditional Chinese medicine-traditional
Chinese rheumatology (ZYXK2012012).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2016]版：
大类|3 区医学
小类|4 区风湿病学
最新[2025]版：
大类|4 区医学
小类|4 区风湿病学
第一作者：
第一作者机构：[1]Department of Rheumatology, Shanghai Guanghua Hospital of Integrated Traditional and Western Medicine, Shanghai, China,
共同第一作者：
通讯作者：
通讯机构：[1]Department of Rheumatology, Shanghai Guanghua Hospital of Integrated Traditional and Western Medicine, Shanghai, China,[2]Arthritis Institute of Integrated Traditional and Western Medicine, Shanghai Chinese Medicine Research Institute, Shanghai, China,[5]Department of Immunology and Microbiology, Shanghai JiaoTong University School of Medicine, Shanghai, China[*1]Department of Rheumatology, Guanghua Integrative Medicine Hospital, Changning District, Shanghai 200052, China.[*2]Department of Immunology and Microbiology, Shanghai JiaoTong University School of Medicine, Shanghai 200025, China.
推荐引用方式(GB/T 7714)：
Shicheng Guo,Qi Zhu,Ting Jiang,et al.Genome-wide DNA methylation patterns in CD4+ T cells from Chinese Han patients with rheumatoid arthritis.[J].Modern rheumatology.2017,27(3):441-447.doi:10.1080/14397595.2016.1218595.
APA：
Shicheng Guo,Qi Zhu,Ting Jiang,Rongsheng Wang,Yi Shen...&Dong Yi He.(2017).Genome-wide DNA methylation patterns in CD4+ T cells from Chinese Han patients with rheumatoid arthritis..Modern rheumatology,27,(3)
MLA：
Shicheng Guo,et al."Genome-wide DNA methylation patterns in CD4+ T cells from Chinese Han patients with rheumatoid arthritis.".Modern rheumatology 27..3(2017):441-447
