机构：[1]School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China[2]Key Laboratory ofBioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing100084, China[3]School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Guangzhou 510006,China[4]Department of Pharmacy and Center for Computational Science and Engineering, National University of Singapore, 117542,Singapore[5]Guang’anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China[6]School of Pharmacy,Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China[7]State Key Laboratory for Quality Research in ChineseMedicines, Macau University of Science and Technology, Macau, China
It is difficult to accurately evaluate the efficacy of traditional Chinese medicine (TCM), which leads to the uncertainty and complexity of dose-effect analysis. In this study we established the "Focus" mode of biomarkers to characterize the dose-effect relationship of Gegen Qinlian Decoction (GQD), a TCM formula for treating type 2 diabetes mellitus (2-DM). A rat model of 2-DM was established through high fat diet feeding combined with low-dose STZ injection. Rats with 2-DM were administered high, middle or low doses (6.785, 4.071, 1.357 mg·kg-1·d-1, respectively) of GQD extract for 60 d. Metformin (300 mg·kg-1·d-1) was taken as the positive control. Blood samples were collected to assess serum biochemical indexes and metabolic profiling. After "Focus" analysis, the biochemical index triglycerides (TG) and insulin sensitivity (ISI) were identified as focused integrated biomarkers (FIBs), while arachidonic acid and docosatetraenoic acid were the metabolic FIBs. Dose-effect relationship curves of GQD were built based on these types of FIBs. Furthermore, the two dose-effect relationship curves showed similar trends with the middle dosage displaying the greatest efficacy, suggesting that insulin function and arachidonic acid metabolism played important roles in 2-DM and the responses to GQD. The metabolic FIB docosatetraenoic should be further explored for understanding its involvement in the process of 2-DM occurrence and the treatment. This "Focus" mode provides a novel strategy to evaluate the dose-effect relationship of a TCM. The system and concepts established here may also be applicable for assessing the dose-effect relationships of Western medicines.
基金：
Chinese National “973” Project
(Grant No 2010CB530600) and the National Natural Science
Foundation of China (Grant No 81130066 and 81302731).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2016]版：
大类|3 区医学
小类|3 区化学综合3 区药学
最新[2025]版：
大类|2 区医学
小类|1 区药学2 区化学：综合
第一作者：
第一作者机构：[1]School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China[2]Key Laboratory ofBioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing100084, China[3]School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Guangzhou 510006,China[4]Department of Pharmacy and Center for Computational Science and Engineering, National University of Singapore, 117542,Singapore
通讯作者：
通讯机构：[1]School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China[3]School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Guangzhou 510006,China[4]Department of Pharmacy and Center for Computational Science and Engineering, National University of Singapore, 117542,Singapore
推荐引用方式(GB/T 7714)：
Huang Zhi-Qiang,Fan Xue-Mei,Wang Yi-Ming,et al.A new method to evaluate the dose-effect relationship of a TCM formula Gegen Qinlian Decoction: "Focus" mode of integrated biomarkers.[J].Acta Pharmacologica Sinica.2017,38(8):1141-1149.doi:10.1038/aps.2016.165.
APA：
Huang Zhi-Qiang,Fan Xue-Mei,Wang Yi-Ming,Liang Qiong-Lin,Tong Xiao-Lin...&Chen Chao.(2017).A new method to evaluate the dose-effect relationship of a TCM formula Gegen Qinlian Decoction: "Focus" mode of integrated biomarkers..Acta Pharmacologica Sinica,38,(8)
MLA：
Huang Zhi-Qiang,et al."A new method to evaluate the dose-effect relationship of a TCM formula Gegen Qinlian Decoction: "Focus" mode of integrated biomarkers.".Acta Pharmacologica Sinica 38..8(2017):1141-1149
