机构：[1]School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong, China[2]Department of Liver Diseases, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen, Guangdong, China深圳市康宁医院深圳医学信息中心[3]Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes,Department of Developmental Biology, Institute of Genetic Engineering, School of Basic Medical Sciences,Southern Medical University, Guangzhou, Guangdong, China[4]The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional ChineseMedicine, Southern Medical University, Guangzhou, Guangdong, China
Alcoholic liver disease (ALD) is a series of abnormalities of liver function, including alcoholic steatosis, steatohepatitis, and cirrhosis. Hesperidin, the major constituent of flavanone in grapefruit, is proved to play a role in antioxidation, anti-inflammation, and reducing multiple organs damage in various animal experiments. However, the underlying mechanism of resistance to alcoholic liver injury is still unclear. Thus, we aimed to investigate the protective effects of hesperidin against ALD and its molecular mechanism in this study. We established an ALD zebrafish larvae model induced by 350 mM ethanol for 32 hours, using wild-type and transgenic line with liver-specific eGFP expression Tg (lfabp10α:eGFP) zebrafish larvae (4 dpf). The results revealed that hesperidin dramatically reduced the hepatic morphological damage and the expressions of alcohol and lipid metabolism related genes, including cyp2y3, cyp3a65, hmgcra, hmgcrb, fasn, and fads2 compared with ALD model. Moreover, the findings demonstrated that hesperidin alleviated hepatic damage as well, which is reflected by the expressions of endoplasmic reticulum stress and DNA damage related genes (chop, gadd45αa, and edem1). In conclusion, this study revealed that hesperidin can inhibit alcoholic damage to liver of zebrafish larvae by reducing endoplasmic reticulum stress and DNA damage, regulating alcohol and lipid metabolism.
基金：
the National Natural Science
Foundation of China (81603501 and 81302948), Science
and Technology Planning Project of Guangzhou City
(201508020014 and 201707010080), Science and Technology
Planning Project of Guangdong Province (2014A020221097),
China Postdoctoral Science Foundation (2016M592508),
Administration of Traditional Chinese Medicine of Guangdong
Province (20162087), and the Scientific Research Initiative
Program of Southern Medical University (LX2015N003,
PY2016N001). The authors thank Miss Haiyan An (Southern
Medical University, Guangzhou, China) for technical assistance.
第一作者机构：[1]School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong, China
共同第一作者：
通讯作者：
通讯机构：[1]School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong, China[4]The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional ChineseMedicine, Southern Medical University, Guangzhou, Guangdong, China
推荐引用方式(GB/T 7714)：
Zhou Zhenting,Zhong Weichao,Lin Haiyan,et al.Hesperidin Protects against Acute Alcoholic Injury through Improving Lipid Metabolism and Cell Damage in Zebrafish Larvae.[J].EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE.2017,2017:doi:10.1155/2017/7282653.
APA：
Zhou Zhenting,Zhong Weichao,Lin Haiyan,Huang Peng,Ma Ning...&Lv Zhiping.(2017).Hesperidin Protects against Acute Alcoholic Injury through Improving Lipid Metabolism and Cell Damage in Zebrafish Larvae..EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2017,
MLA：
Zhou Zhenting,et al."Hesperidin Protects against Acute Alcoholic Injury through Improving Lipid Metabolism and Cell Damage in Zebrafish Larvae.".EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2017.(2017)
