机构：[1]Department of Oral and Maxillofacial Surgery, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong, 510080, China中山大学附属第一医院[2]Department of Oral and Maxillofacial Surgery, Afliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi Province, 330006, China[3]School of Stomatology, Nanchang University, Nanchang, Jiangxi Province, 330006, China.
Oleic acid (OA), a main ingredient of Brucea javanica oil (BJO), is widely known to have anticancer effects in many tumors. In this study, we investigated the anticancer effect of OA and its mechanism in tongue squamous cell carcinoma (TSCC). We found that OA effectively inhibited TSCC cell proliferation in a dose- and time-dependent manner. OA treatment in TSCC significantly induced cell cycle G0/G1 arrest, increased the proportion of apoptotic cells, decreased the expression of CyclinD1 and Bcl-2, and increased the expression of p53 and cleaved caspase-3. OA also obviously induced the formation of autolysosomes and decreased the expression of p62 and the ratio of LC3 I/LC3 II. The expression of p-Akt, p-mTOR, p-S6K, p-4E-BP1 and p-ERK1/2 was significantly decreased in TSCC cells after treatment with OA. Moreover, tumor growth was significantly inhibited after OA treatment in a xenograft mouse model. The above results indicate that OA has a potent anticancer effect in TSCC by inducing apoptosis and autophagy via blocking the Akt/mTOR pathway. Thus, OA is a potential TSCC drug that is worthy of further research and development.
基金：
National Nature Science Foundation of China
(NSFC81672659, NSFC81472523, NSFC81272953), the Guangdong Natural Science Foundation
(2015A030313017), the Guangzhou Science and Technology Project (Collaborative Innovation Major Projects,
201605131226218), the Jiangxi Province Health Department of Science and Technology Plan (20091067), and
the Science and Technology Research Project from the Jiangxi Province Department of Education (GJJ11330).
第一作者机构：[1]Department of Oral and Maxillofacial Surgery, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong, 510080, China[2]Department of Oral and Maxillofacial Surgery, Afliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi Province, 330006, China[3]School of Stomatology, Nanchang University, Nanchang, Jiangxi Province, 330006, China.
共同第一作者：
通讯作者：
通讯机构：[2]Department of Oral and Maxillofacial Surgery, Afliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi Province, 330006, China[3]School of Stomatology, Nanchang University, Nanchang, Jiangxi Province, 330006, China.
推荐引用方式(GB/T 7714)：
Jiang Lin,Wang Wei,He Qianting,et al.Oleic acid induces apoptosis and autophagy in the treatment of Tongue Squamous cell carcinomas.[J].SCIENTIFIC REPORTS.2017,7:doi:10.1038/s41598-017-11842-5.
APA：
Jiang Lin,Wang Wei,He Qianting,Wu Yuan,Lu Zhiyuan...&Wang Anxun.(2017).Oleic acid induces apoptosis and autophagy in the treatment of Tongue Squamous cell carcinomas..SCIENTIFIC REPORTS,7,
MLA：
Jiang Lin,et al."Oleic acid induces apoptosis and autophagy in the treatment of Tongue Squamous cell carcinomas.".SCIENTIFIC REPORTS 7.(2017)
