机构：[1]Department of Orthopaedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710004, Shaanxi Province, P.R. China[2]Department of Orthopaedics, Shaanxi Provincial People’s Hospital, The Third Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710068, Shaanxi Province, P.R. China[3]Department of Oncology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710061, Shaanxi Province, P.R. China[4]Department of Surgical Oncology, Shaanxi Provincial People’s Hospital, The Third Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710068, Shaanxi Province, P.R. China[5]Department of Clinical Microbiology and Infection Control, The University of Hong Kong - Shenzhen Hospital, Shenzhen, 518053, Guangdong Province, P.R. China深圳市康宁医院深圳医学信息中心香港大学深圳医院[6]Department of Gynecology and Obstetrics, The Second Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang, 712000, Shaanxi Province, P.R. China
Accumulating evidence indicates that dysregulation of miRNAs could contribute to tumor growth and metastasis of chondrosarcoma by infuencing cell proliferation and invasion. In the current study, we are interested to examine the role of miRNAs in the carcinogenesis and progression of chondrosarcoma. Here, using comparative miRNA profiling of tissues and cells of chondrosarcoma and cartilage, we identified miR-494 as a commonly downregulated miRNA in the tissues of patients with chondrosarcoma and chondrosarcoma cancer cell line, and upregulation of miR-494 could inhibit proliferation and invasion of chondrosarcoma cancer cells in vivo and in vitro. Moreover, our data demonstrated that SOX9, the essential regulator of the process of cartilage differentiation, was the direct target and functional mediator of miR-494 in chondrosarcoma cells. And downregulation of SOX9 could also inhibit migration and invasion of chondrosarcoma cells. In the last, we identified low expression of miR-494 was significantly correlated with poor overall survival and prognosis of chondrosarcoma patients. Thus, miR-494 may be a new common therapeutic target and prognosis biomarker for chondrosarcoma.
基金：
the National Natural Science Foundation of China (No. 81371944).
第一作者机构：[1]Department of Orthopaedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710004, Shaanxi Province, P.R. China[2]Department of Orthopaedics, Shaanxi Provincial People’s Hospital, The Third Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710068, Shaanxi Province, P.R. China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Li Jingyuan,Wang Lijuan,Liu Zongzhi,et al.MicroRNA-494 inhibits cell proliferation and invasion of chondrosarcoma cells in vivo and in vitro by directly targeting SOX9.[J].ONCOTARGET.2015,6(28):26216-26229.doi:10.18632/oncotarget.4460.
APA：
Li Jingyuan,Wang Lijuan,Liu Zongzhi,Zu Chao,Xing Fanfan...&Wang Kunzheng.(2015).MicroRNA-494 inhibits cell proliferation and invasion of chondrosarcoma cells in vivo and in vitro by directly targeting SOX9..ONCOTARGET,6,(28)
MLA：
Li Jingyuan,et al."MicroRNA-494 inhibits cell proliferation and invasion of chondrosarcoma cells in vivo and in vitro by directly targeting SOX9.".ONCOTARGET 6..28(2015):26216-26229
