机构：[1]Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, No. 1838, Guangzhou 510515, China[2]The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China[3]Dean’s Office, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China[4]Hygiene Detection Center, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China[5]Traditional Chinese Medicine Integrated Hospital, Southern Medical University, Guangzhou, Guangdong 510305, China
Xiao Yao San (XYS) is a classical Chinese medicine formula that has been widely used to treat mood disorders for hundreds of years. To confirm the effect of XYS and better understand its underlying mechanism, high-performance liquid chromatography-mass spectrometry analysis-based quality control of XYS extracts and proteomics-based identification of differential proteins in the hippocampus were adopted in social isolation and chronic unpredictable mild stress- (CUMS-) treated rats. The depressive-like behavior of rats induced by CUMS resembled the manifestation of human depression. The upregulated corticosterone (CORT) and urocortin 2 (UCN2) levels demonstrated the existence of hypothalamic-pituitary-adrenal (HPA) axis hyperactivity. XYS was effective in ameliorating the depressive-like behavior and downregulating UCN2 and CORT. XYS decreased the expression of serine/threonine-protein phosphatase 2A subunit B and increased the expression of β-arrestin 2. The expressions of brain-derived neurotrophic factor (BDNF), tyrosine receptor kinase B (TrkB), and mammalian target of rapamycin (mTOR) were also elevated by XYS. In conclusion, XYS improves social isolation and CUMS-induced depressive-like behavior and ameliorates HPA hyperactivation through the downregulation of corticotrophin releasing hormone (CRH) receptor 2. The upregulation of BDNF/TrkB and the phosphorylation of mTOR require β-arrestin 2 as a scaffold to regulate stress signaling.
基金：
National Natural Sciences Foundation of China (nos. 81173168 and 81230085);Science and Technology Project of Guangzhou (no.
12C32121551); Construction Traditional Chinese Medicine
Province Research Projects of TCM Bureau of Guangdong
Province (20121121)
第一作者机构：[1]Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, No. 1838, Guangzhou 510515, China[2]The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, No. 1838, Guangzhou 510515, China[2]The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China
推荐引用方式(GB/T 7714)：
Zhu Xiaoxia,Xia Oudong,Han Weili,et al.Xiao Yao San Improves Depressive-Like Behavior in Rats through Modulation of β-Arrestin 2-Mediated Pathways in Hippocampus.[J].EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE.2014,2014:doi:10.1155/2014/902516.
APA：
Zhu Xiaoxia,Xia Oudong,Han Weili,Shao Meng,Jing Linlin...&Sun Xuegang.(2014).Xiao Yao San Improves Depressive-Like Behavior in Rats through Modulation of β-Arrestin 2-Mediated Pathways in Hippocampus..EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2014,
MLA：
Zhu Xiaoxia,et al."Xiao Yao San Improves Depressive-Like Behavior in Rats through Modulation of β-Arrestin 2-Mediated Pathways in Hippocampus.".EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2014.(2014)
