机构：[1]School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515[2]Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, Guangdong 510010[3]Department of Pharmacy, Hunan Loudi Central Hospital, Loudi, Hunan 417000, P.R. China
Aloperine (ALO) is a quinolizidine alkaloid extracted from the leaves of Sophora alopecuroides (S. alopecuroides) and possesses anti-inflammatory, anti-allergenic, antitumor, and antiviral effects. In this study, when compared with seven other types of alkaloids extracted from S. alopecuroides, ALO treatment produced the most potent effects against HCT116 colon cancer cell types. ALO inhibited proliferation and induced apoptosis in HCT116 cells in a dose- and time-dependent manner as detected by MTT, clonogenic survival, and flow cytometric assays. Results of the western blot analysis and qPCR revealed that ALO increased the protein and mRNA of Bax and decreased Bcl-2 via the mitochondrial death pathway. In addition, ALO induced cell cycle arrest at the G2/M phase with a concomitant increase in p21 and p53 and a decrease in cyclin D1 and B1. ALO also inhibited phosphatidylinositol 3-kinase/Akt and JAK/Stat3. Generally, ALO exerted a significant anti-proliferative effect via apoptotic and cell cycle arrest induction in HCT116 cells. These results suggested that ALO should be investigated further as an agent of chemotherapeutic activity in human colon cancer.
基金：
the Natural Science Foundation of China (no. 81001701 to Dr L.L.); the Project of Zhujiang New Star of Guangzhou Science and Technology Project (no. 2013J2200030 to Dr L.L.).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2013]版：
大类|4 区医学
小类|4 区医学：研究与实验
最新[2025]版：
大类|2 区医学
小类|2 区医学：研究与实验
第一作者：
第一作者机构：[1]School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515
共同第一作者：
通讯作者：
通讯机构：[1]School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515[*1]School of Traditional Chinese Medicine, Southern Medical University, 1023 Shatai Southern Road, Guangzhou, Guangdong 510515, P.R. China
推荐引用方式(GB/T 7714)：
Zhang Li,Zheng Yanxin,Deng Hongzhu,et al.Aloperine induces G2/M phase cell cycle arrest and apoptosis in HCT116 human colon cancer cells.[J].International journal of molecular medicine.2014,33(6):1613-20.doi:10.3892/ijmm.2014.1718.
APA：
Zhang Li,Zheng Yanxin,Deng Hongzhu,Liang Lei&Peng Juan.(2014).Aloperine induces G2/M phase cell cycle arrest and apoptosis in HCT116 human colon cancer cells..International journal of molecular medicine,33,(6)
MLA：
Zhang Li,et al."Aloperine induces G2/M phase cell cycle arrest and apoptosis in HCT116 human colon cancer cells.".International journal of molecular medicine 33..6(2014):1613-20
