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Cationic micellar nanoparticles for DNA and doxorubicin co-delivery.

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机构: [a]Traditional Chinese Medicine and New Drug Research Institute, Guangdong Medical College, Dongguan 523808, China [b]Department of Traditional Chinese Medicine, The Second Clinical Medical College, Guangdong Medical College, Dongguan 523808, China [c]Department of Chemistry, Virginia Tech, Blacksburg, VA 24061, United States [d]Department of Microbiology and Immunology, School of Basic Medicine, Guangdong Medical College, Dongguan 523808, China [e]Emergency Department, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, China [f]DSAPM Lab, PCFM Lab, Institute of Polymer Science, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China
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Cationic micellar nanoparticles for chemotherapeutic drugs and therapeutic gene co-delivery were prepared based on a poly-(N-ε-carbobenzyloxy-l-lysine) (PZLL) and dendritic polyamidoamine (PAMAM) block copolymer (PZLL-D3). PZLL-D3 was synthesized by a copper-catalyzed azide alkyne cyclization (click) reaction between α-alkyne-PZLL and azide focal point PAMAM dendrons. Its structure was characterized by (1)H NMR and FTIR, and its buffering capability was determined by acid-base titration. MTT, agarose gel electrophoresis and flow cytometry studies showed that PZLL-D3 revealed low in vitro cytotoxicity, strong pDNA condensation ability, protection of pDNA against deoxyribonuclease I degradation and high gene transfection efficiency in 293T and HeLa cells. In addition, the micellar nanoparticles delivered pDNA and anticancer drug doxorubicin (DOX) simultaneously and efficiently to tumor cells, and the DOX loaded nanoparticles showed sustained in vitro release at pH=7.4 and 5.8. Copyright © 2014. Published by Elsevier B.V.

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大类 | 2 区 工程技术
小类 | 3 区 材料科学:生物材料
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第一作者机构: [a]Traditional Chinese Medicine and New Drug Research Institute, Guangdong Medical College, Dongguan 523808, China
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