Asarone from Acori Tatarinowii Rhizoma Potentiates the Nerve Growth Factor-Induced Neuronal Differentiation in Cultured PC12 Cells: A Signaling Mediated by Protein Kinase A.
机构：[1]Division of Life Science, Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.[2]Shenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen, China.[3]HKUST Shenzhen Research Institute, Hi-Tech Park, Nanshan, Shenzhen, Guangdong Province, China.
Acori Tatarinowii Rhizoma (ATR), the rhizome of Acorus tatarinowii Schott, is being used clinically to treat neurological disorders. The volatile oil of ATR is being considered as an active ingredient. Here, α-asarone and β-asarone, accounting about 95% of ATR oil, were evaluated for its function in stimulating neurogenesis. In cultured PC12 cells, application of ATR volatile oil, α-asarone or β-asarone, stimulated the expression of neurofilaments, a bio-marker for neurite outgrowth, in a concentration-dependent manner. The co-treatment of ATR volatile oil, α-asarone or β-asarone, with low concentration of nerve growth factor (NGF) potentiated the NGF-induced neuronal differentiation in cultured PC12 cells. In addition, application of protein kinase A inhibitors, H89 and KT5720, in cultures blocked the ATR-induced neurofilament expression, as well as the phosphorylation of cAMP-responsive element binding protein (CREB). In the potentiation of NGF-induced signaling in cultured PC12 cells, α-asarone and β-asarone showed synergistic effects. These results proposed the neurite-promoting asarone, or ATR volatile oil, could be useful in finding potential drugs for treating various neurodegenerative diseases, in which neurotrophin deficiency is normally involved.
基金：
This research was supported by Hong Kong Research Grants Council Theme-based Research
Scheme (T13-607/12R), GRF (663012, 662713, M-HKUST604/13), TUYF15SC01, The
Hong Kong Jockey Club Charities Trust (HKJCCT12SC01) and Foundation of The Awareness
of Nature (TAON12SC01) to Karl TSIM, JCYJ20160229205726699 to Prof. Tsim,
JCYJ20160229205812004 to Dr. Dong and JCYJ20160229210027564 to Dr. Wang. Kelly Y.C.
LAM received a Kanya Lee Scholarship from Lee's Pharmaceutical Co. Ltd.
第一作者机构：[1]Division of Life Science, Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.
通讯作者：
通讯机构：[1]Division of Life Science, Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.[3]HKUST Shenzhen Research Institute, Hi-Tech Park, Nanshan, Shenzhen, Guangdong Province, China.
推荐引用方式(GB/T 7714)：
Lam Kelly Y C,Chen Jianping,Lam Candy T W,et al.Asarone from Acori Tatarinowii Rhizoma Potentiates the Nerve Growth Factor-Induced Neuronal Differentiation in Cultured PC12 Cells: A Signaling Mediated by Protein Kinase A.[J].PLOS ONE.2016,11(9):doi:10.1371/journal.pone.0163337.
APA：
Lam Kelly Y C,Chen Jianping,Lam Candy T W,Wu Qiyun,Yao Ping...&Tsim Karl W K.(2016).Asarone from Acori Tatarinowii Rhizoma Potentiates the Nerve Growth Factor-Induced Neuronal Differentiation in Cultured PC12 Cells: A Signaling Mediated by Protein Kinase A..PLOS ONE,11,(9)
MLA：
Lam Kelly Y C,et al."Asarone from Acori Tatarinowii Rhizoma Potentiates the Nerve Growth Factor-Induced Neuronal Differentiation in Cultured PC12 Cells: A Signaling Mediated by Protein Kinase A.".PLOS ONE 11..9(2016)
