机构：[1]Department of Traditional Chinese Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong, China[2]Syndrome Laboratory of Integrated Chinese and Western Medicine, School of Chinese Medicine, Southern Medical University, Guangzhou, Guangdong, China[3]Department of Oncology, Affiliated Dongguan People’s Hospital, Southern Medical University, Guangzhou, Guangdong, China[4]Traditional Chinese Pharmacological Laboratory, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong, China[5]School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China
Heart, liver, and kidney, which are known as the essential organs for metabolism, possess the unique ability to regulate the proliferation function of the body against injury. Silibinin (SB), a natural polyphenolic flavonoid extracted from traditional herb Silybum marianum L., has been used to protect hepatocytes. Whether SB can regulate mitochondrial fission in normal cells and the underlying mechanisms remain unclear. Here, we showed that SB markedly promoted cell proliferation by facilitating G1/S transition via activating dynamin-related protein 1 (Drp1), which in turn mediated mitochondrial fission in these normal cells. SB dose-dependently increased the mitochondrial mass, mtDNA copy number, cellular adenosine triphosphate production, mitochondrial membrane potential, and reactive oxygen species in normal cells. Furthermore, SB dose-dependently increased the expression of Drp1. Blocking Drp1 abolished SB-induced mitochondrial fission. In conclusion, we demonstrate that SB promotes cell proliferation through facilitating G1/S transition by activating Drp1-mediated mitochondrial fission. This study suggests that SB is a potentially useful herbal derivative for the daily prevention of various diseases caused by impaired mitochondrial fission.
基金：
The author(s) disclosed receipt of the following financial support for
the research, authorship, and/or publication of this article: This work
was supported by the Key Project of National Natural Science Foundation
of China (No. 81830117), the National Science Foundation of
China (Nos. 81673840, 819040037), the Natural Science Foundation
of Guangdong Province, China (Nos. 2017A030313791,
2017A030313722, 2018A030313375), and the Project of Administration
of Traditional Chinese Medicine of Guangdong Province of
China (No. 20181172).
语种：
外文
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最新[2025]版：
大类|4 区医学
小类|3 区移植4 区细胞与组织工程4 区医学：研究与实验
第一作者：
第一作者机构：[1]Department of Traditional Chinese Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong, China[2]Syndrome Laboratory of Integrated Chinese and Western Medicine, School of Chinese Medicine, Southern Medical University, Guangzhou, Guangdong, China
共同第一作者：
通讯作者：
通讯机构：[2]Syndrome Laboratory of Integrated Chinese and Western Medicine, School of Chinese Medicine, Southern Medical University, Guangzhou, Guangdong, China[*1]Syndrome Laboratory of Integrated Chinese and Western Medicine, School of Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China
推荐引用方式(GB/T 7714)：
You Yanting,Chen Liqian,Wu Yifen,et al.Silibinin Promotes Cell Proliferation Through Facilitating G1/S Transitions by Activating Drp1-Mediated Mitochondrial Fission in Cells.[J].Cell transplantation.29:963689720950213.doi:10.1177/0963689720950213.
APA：
You Yanting,Chen Liqian,Wu Yifen,Wang Ming,Lu Hanqi...&Dai Jiaojiao.(0).Silibinin Promotes Cell Proliferation Through Facilitating G1/S Transitions by Activating Drp1-Mediated Mitochondrial Fission in Cells..Cell transplantation,29,
MLA：
You Yanting,et al."Silibinin Promotes Cell Proliferation Through Facilitating G1/S Transitions by Activating Drp1-Mediated Mitochondrial Fission in Cells.".Cell transplantation 29.(0):963689720950213
