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The effects of interferon transcription factor 3 on apoptosis and proliferation of lx-2 cells through positive regulation of akt signaling pathway

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机构： [1]Department of the 2nd Surgery, GuangDong Second Traditional Chinese Medicine Hospital, Guangzhou, China [2]Department of Anesthesiology, GuangDong Second Traditional Chinese Medicine Hospital, Guangzhou, China [3]Department of Medical Technology, GuangDong Second Traditional Chinese Medicine Hospital, Guangzhou, China

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关键词： AKT signalling pathway Interferon transcription factor 3 LX-2 cells

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Objective: To investigate the effect of interferon transcription factor 3 (IRF3) on the apoptosis and proliferation of LX-2 cells through positive regulation of the serine threonine kinase (AKT) signalling pathway. Methods: LX-2 cells were collected for high glucose culture. After RNAi interference, plasmid extraction and transfection, the empty vector pcDNA3 group (control) and plasmid pcDNA3-IRF3 group (plasmid pcDNA3-IRF3 were transferred into LX-2 cells and over-expressed IRF3), TGFβ-1 group (LX-2 cells induced activation by TGFβ-1), siRNA-IRF3 group (transfected siRNA-IRF3 into LX-2 cells), and IRF3-NC group (negative control). The researchers observed the effect of silencing IRF3 expression on the proliferation and apoptosis of LX-2 cells and the effect of overexpression and silencing of IRF3 on the apoptosis-related protein Caspase-3, BAX, and B-cell lymphoma factor 2 (Bcl-2). The effect of the expression and silencing of IRF3 on the AKT signalling pathway was further analysed. Results: The inhibition of LX-2 cell proliferation in the IRF3-siRNA transfected group was significantly stronger than that in the IRF3-NC group (P0.05). The promotion of apoptosis by the IRF3-siRNA group was significantly stronger than that of the IRF3-NC group (P0.01). The expression of Cleave-Caspase-3 and BAX in the IRF3-siRNA group was significantly higher than in the IRF3-NC group, and the expression of Bcl-2 was significantly lower than in the IRF3-NC group (P0.05). The expression of Cleave-Caspase-3 and BAX in the pcDNA3-IRF3 group was significantly higher than in the pcDNA3 group, and the expression of Bcl-2 was significantly lower than in the pcDNA3-IRF3 group (P0.05). There was no significant difference in the expression of AKT between the IRF-siRNA group and the IRF3-NC group (P0.05). The expression of p-AKT in the IRF3-siRNA group was significantly lower than in the IRF3-NC group (P0.05). There was no significant difference in the expression of AKT between the pcDNA3-IRF3 group and the pcDNA3 group (P0.05). The expression of p-AKT in the pcDNA3-IRF3 group was significantly higher than in the pcDNA3 group (P0.05). Conclusion: IRF3 can regulate the apoptosis and proliferation of LX-2 cells by positively regulating the AKT signalling pathway, which may become a key target in anti-fibrosis treatment. © 2020 A. CARBONE Editore. All rights reserved.

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大类 | 4 区医学

小类 | 4 区医学：内科

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第一作者机构： [1]Department of the 2nd Surgery, GuangDong Second Traditional Chinese Medicine Hospital, Guangzhou, China

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