Ovarian cancer (OC) remains the first leading cause of gynecologic malignancy. The survival of Serous Ovarian Cancer (SOC) is poor and the present prognostic predictors of SOC are not very sensitive or specific. The present study aimed to investigate Microtubule-Actin Cross-Linking Factor 1 (MACF1) expression in SOC tissues (including paraffin-embedded and fresh tissues) and to assess its expression and significant value in patients with SOC. A total of 18 fresh SOC tissues and their paired paratumor tissues were performed with reverse-transcription quantitative PCR analysis to detect MACF1 mRNA expression. Moreover, 175 paraffin embedded SOC tissues and 41 paratumor tissues were assessed for MACF1 expression using immunohistochemistry. The mRNA and protein expression of MACF1 were both higher in cancer tissues than that in paratumor tissues, and MACF1 high expression was associated with shorter Recurrence Free Survival (RFS) and Overall Survival (OS) in patients with SOC. Furthermore, multivariate regression analysis showed that high MACF1 expression was an independent poor survival predictor of patients with SOC. MACF1 is upregualted in SOC, and it may be used as a useful patent of prognostic biomarker in SOC. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.