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COVID-19 immune features revealed by a large-scale single-cell transcriptome atlas.

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收录情况: ◇ SCIE ◇ 自然指数

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机构: [1]Biomedical Pioneering Innovation Center (BIOPIC), School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Academy forAdvanced Interdisciplinary Studies, Beijing Advanced Innovation Center for Genomics (ICG), Peking University, Beijing 100871, China [2]National Center for Liver Cancer, Second Military Medical University, Shanghai 200003, China [3]Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200003, China [4]Ministry of Education (MOE) Key Laboratory on Signaling Regulation and Targeting Therapy of Liver Cancer, Second Military MedicalUniversity, Shanghai 200003, China [5]Bioland Laboratory (Guangzhou Regenerative Medicine and Health GuangDong Laboratory), Guangzhou 510005, China [6]Institute of Cancer Research, Shenzhen Bay Laboratory, Shenzhen 518132, China [7]Beijing Youan Hospital, Capital Medical University, Beijing 100069, China [8]Department of Oncology, The First Affiliated Hospital of USTC, Division of Molecular Medicine, Hefei National Laboratory for PhysicalSciences at Microscale, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230021, China [9]Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, The Second AffiliatedHospital, School of Medicine, Southern University of Science and Technology, Shenzhen 518112, China [10]Center for Life Sciences, School of Life Science and Technology, Harbin Institute of Technology, 150080 Harbin, China [11]State Key Laboratory of Brain and Cognitive Science, CAS Center for Excellence in Brain Science and Intelligence Technology, Institute ofBrain-Intelligence Technology (Shanghai), Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China [12]State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University CancerCenter, Guangzhou 510060, China [13]Center for Bioinformatics, School of Life Science and Technology, Harbin Institute of Technology, China [14]State Key Laboratory of Experimental Hematology and National Clinical Research Center for Blood Diseases, Institute of Hematology andBlood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China [15]Center for Stem Cell Medicine and Department of Stem Cell & Regenerative Medicine, Chinese Academy of Medical Sciences and PekingUnion Medical College, Tianjin 300020, China [16]Department of Infectious Diseases, Southwest Hospital, Army Medical University, Chongqing 400038, China [17]State Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, Beijing 100875, China [18]Analytical Biosciences Beijing Limited, Beijing 100084, China [19]State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China [20]Guangzhou Eighth People’s Hospital, Guangzhou Medical University, Guangzhou 510060, China [21]State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of RespiratoryHealth, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510120, China [22]Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China [23]Department of Infectious Disease, Shanghai Public Health Clinical Center, Shanghai 201052, China [24]Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Army Medical University, Chongqing 400038, China [25]Cancer Center, Renmin Hospital of Wuhan University, Wuhan 430060, China [26]Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030,Hubei, China [27]Shanghai Institute of Immunology, Department of Microbiology and Immunology, Shanghai Jiao Tong University School of Medicine,Shanghai 200025, China [28]Department of Infectious Diseases, Fifth Medical Center of Chinese PLA General Hospital, National Clinical Research Center for InfectiousDiseases, Beijing 100039, China [29]Cancer Center, Huanggang Hospital of Traditional Chinese Medicine, Huanggang 438000, China [30]Center for Infection and Immunity, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, Guangdong 519000, China [31]Cancer Center, Renmin Hospital of Wuhan University, Wuhan 430060, China [32]Department of Laboratory Medicine, Yuebei People’s Hospital, Shantou University Medical College, Shaoguan 512025, China [33]Laboratory for Diagnosis of Clinical Microbiology and Infection, Medical Research Center, Shantou University Medical College, Shaoguan512025, China [34]Center for Quantitative Biology, Peking University, Beijing 100871, China [35]Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China [36]Shanghai Public Health Clinical Center and Institute of Biomedical Sciences, Fudan University, Shanghai 201508, China [37]Yuebei People’s Hospital, Shantou University Medical College, Shaoguan 512025, China [38]Intelligent Pathology Institute, Division of Life Sciences and Medicine, University of Science and Technology of China (USTC), andDepartment of Pathology, the First Hospital Affiliated to USTC, Hefei, Anhui 230036, China [39]Department of Pathology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
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摘要:
A dysfunctional immune response in coronavirus disease 2019 (COVID-19) patients is a recurrent theme impacting symptoms and mortality, yet a detailed understanding of pertinent immune cells is not complete. We applied single-cell RNA sequencing to 284 samples from 196 COVID-19 patients and controls and created a comprehensive immune landscape with 1.46 million cells. The large dataset enabled us to identify that different peripheral immune subtype changes are associated with distinct clinical features, including age, sex, severity, and disease stages of COVID-19. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA was found in diverse epithelial and immune cell types, accompanied by dramatic transcriptomic changes within virus-positive cells. Systemic upregulation of S100A8/A9, mainly by megakaryocytes and monocytes in the peripheral blood, may contribute to the cytokine storms frequently observed in severe patients. Our data provide a rich resource for understanding the pathogenesis of and developing effective therapeutic strategies for COVID-19. Copyright © 2021 Elsevier Inc. All rights reserved.

基金编号: 2020YFC0848700 2016YFA0100600 CSTC2020jscx-fyzxX0037 2017YFA0102900 91940306 81788101 31970858 32022016 31771428 91640113 31700796 81871479 81772165 81974303 31991171 31530036 91742203 31825008 31422014 61872117 61822108 62032007 BJYAYY-2020PY-01 Z201100005420018 Z201100005420022 2017-I2M-1-015 YD2070002019 RS202001

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出版当年[2020]版:
大类 | 1 区 生物
小类 | 1 区 生化与分子生物学 1 区 细胞生物学
最新[2025]版:
大类 | 1 区 生物学
小类 | 1 区 生化与分子生物学 1 区 细胞生物学
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出版当年[2019]版:
Q1 CELL BIOLOGY Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
最新[2023]版:
Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Q1 CELL BIOLOGY

影响因子: 最新[2023版] 最新五年平均 出版当年[2019版] 出版当年五年平均 出版前一年[2018版] 出版后一年[2020版]

第一作者:
第一作者机构: [1]Biomedical Pioneering Innovation Center (BIOPIC), School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Academy forAdvanced Interdisciplinary Studies, Beijing Advanced Innovation Center for Genomics (ICG), Peking University, Beijing 100871, China
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通讯作者:
通讯机构: [1]Biomedical Pioneering Innovation Center (BIOPIC), School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Academy forAdvanced Interdisciplinary Studies, Beijing Advanced Innovation Center for Genomics (ICG), Peking University, Beijing 100871, China [2]National Center for Liver Cancer, Second Military Medical University, Shanghai 200003, China [3]Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200003, China [4]Ministry of Education (MOE) Key Laboratory on Signaling Regulation and Targeting Therapy of Liver Cancer, Second Military MedicalUniversity, Shanghai 200003, China [5]Bioland Laboratory (Guangzhou Regenerative Medicine and Health GuangDong Laboratory), Guangzhou 510005, China [6]Institute of Cancer Research, Shenzhen Bay Laboratory, Shenzhen 518132, China [11]State Key Laboratory of Brain and Cognitive Science, CAS Center for Excellence in Brain Science and Intelligence Technology, Institute ofBrain-Intelligence Technology (Shanghai), Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China [14]State Key Laboratory of Experimental Hematology and National Clinical Research Center for Blood Diseases, Institute of Hematology andBlood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China [15]Center for Stem Cell Medicine and Department of Stem Cell & Regenerative Medicine, Chinese Academy of Medical Sciences and PekingUnion Medical College, Tianjin 300020, China [22]Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China [24]Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Army Medical University, Chongqing 400038, China [32]Department of Laboratory Medicine, Yuebei People’s Hospital, Shantou University Medical College, Shaoguan 512025, China [33]Laboratory for Diagnosis of Clinical Microbiology and Infection, Medical Research Center, Shantou University Medical College, Shaoguan512025, China [38]Intelligent Pathology Institute, Division of Life Sciences and Medicine, University of Science and Technology of China (USTC), andDepartment of Pathology, the First Hospital Affiliated to USTC, Hefei, Anhui 230036, China
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