机构：[1]Department of Medical Ultrasonic, The Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, P.R. China[2]Department of Pharmacy, The Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, P.R. China[3]Department of Traditional Chinese Medicine, The Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, P.R. China[4]Laboratory of Molecular Immunology, Department of Microbiology and Immunology, Rega Institute, Leuven, Belgium[5]Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital, University of Sun Yat-Sen, Zhuhai, P.R. China
Objectives It has been demonstrated that artemisinin (ART) possesses multiple immune modulatory effects. However, its role as immunosuppressant in allogeneic transplantation is undetermined. Here, we investigated the effect of ART on co-stimulatory signaling in OX40(+) T cells and evaluated ART as a potential immunosuppressant in transplantation. Materials and methods Allogeneic skin transplantation was performed in C57BL/6 to BALB/c mice. Recipient mice were administrated with vehicle, ART or cyclosporine A daily from day 0 to day 19 post transplantation. Proportions of splenic CD4(+)OX40(+) and CD4(+)CD44(hi)CD62L(hi) cells, and serum IgG was measured by using flow cytometry. An in vitro lymphocyte stimulation with Con A or LPS under various concentrations of ART was performed, expression of CD4(+)OX40(+) and CD4(+)CD44(hi)CD62L(hi) cells was evaluated, and interleukin(IL)-6 production was measured by ELISA. Results In in vivo allogeneic skin transplant model, ART significantly prolongs allogeneic skin survival. Furthermore, our in vitro studies demonstrate that the immune suppression of ART on T cells is associated with a reduction in OX40(+) T cells and inhibition of IL-6 secretion. Conclusion Our data indicate that the OX40-OX40L pathway and IL-6 are possibly involved in ART-induced immunosuppression, and ART is a potential novel immunosuppressant.
基金：
Sun Yat-Sen University [Talent Program
2016] and The High Level Health Team on Innovation Research of
Zhuhai [2018].
第一作者机构：[1]Department of Medical Ultrasonic, The Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, P.R. China
共同第一作者：
通讯作者：
通讯机构：[3]Department of Traditional Chinese Medicine, The Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, P.R. China[5]Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital, University of Sun Yat-Sen, Zhuhai, P.R. China[*1]Department of Traditional Chinese Medicine, The Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai 519000, P.R. China
推荐引用方式(GB/T 7714)：
Liu Lihua,Zhao Juanzhi,Li An,et al.Prolongation of allograft survival by artemisinin treatment is associated with blockade of OX40-OX40L[J].IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY.2021,doi:10.1080/08923973.2021.1902347.
APA：
Liu, Lihua,Zhao, Juanzhi,Li, An,Yang, Xuan,Sprangers, Ben&Li, Shengqiao.(2021).Prolongation of allograft survival by artemisinin treatment is associated with blockade of OX40-OX40L.IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY,,
MLA：
Liu, Lihua,et al."Prolongation of allograft survival by artemisinin treatment is associated with blockade of OX40-OX40L".IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY .(2021)
