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Cytotoxicity of adducts formed between quercetin and methylglyoxal in PC-12 cells.

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机构: [a]School of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong 510641, China [b]Department of Hematology, Guangdong Second Provincial General Hospital, Guangzhou, Guangdong 510317, China [c]School of Traditional Chinese Medicine, Jinan University, Guangzhou 510632, China [d]Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China [e]Department of Biochemistry and Molecular Biology, Medical College of Jinan University, Guangzhou 510632, China [f]Formula-pattern Research Center, School of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong 510632, China
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Quercetin (Que) or quercetin-containing food stuffs are widely incorporated in bakery foods for improving food texture and health effects, and scavenging reactive aldehydes, such as methylglyoxal (MGO) that exhibits various deleterious effects including contribution to neurodegeneration. This study aimed to investigate the cytotoxicity of the adducts formed between quercetin and MGO resulted from the incorporation of quercetin in foods. Two highly-purified adducts (Que-mono-MGO and Que-di-MGO) were found to display higher cytotoxicity than their precursor MGO and quercetin. They elevated apoptosis via upregulation of expression of apoptotic markers, including p-P38, cleaved caspase-9 and -3, and pro-apoptotic Bax. They induced mitochondrial dysfunction via decreasing mitochondrial membrane potential and increasing lactate dehydrogenase release. Moreover, they attenuated levels of p-Akt, Nrf2, NQO-1, and HO-1, proving that they induced neurodegeneration apoptosis through mitochondria-mediated signaling pathways (PI3K-Akt and Nrf2-HO-1/NQO-1). These findings indicated that the safety consequence of MGO after scavenged by polyphenols needs to be concerned. Copyright © 2021. Published by Elsevier Ltd.

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出版当年[2020]版:
大类 | 2 区 工程技术
小类 | 1 区 应用化学 1 区 食品科技 1 区 营养学
最新[2025]版:
大类 | 1 区 农林科学
小类 | 1 区 应用化学 1 区 食品科技 1 区 营养学
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第一作者机构: [a]School of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong 510641, China
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