机构：[1]Acupuncture Department, Beijing Shijitan Hospital, Capital Medical University, Beijing, China[2]Ninth School of Clinical Medicine, Peking University, Beijing, China[3]Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, China[4]Clinical Medical College of Acupuncture, Moxibustion and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China深圳市中医院深圳医学信息中心
Zucker diabetic fatty (ZDF) rats that harbor a mutation in the leptin receptor innately develop type 2 diabetes (T2D) with obesity. Transcutaneous auricular vagal nerve stimulation (taVNS) has an antidiabetic effect in ZDF rats. However, the underlying mechanisms of the weight-gain attenuating effect in ZDF rats by taVNS is still unclear. This study aimed to assess whether the weight-gain attenuating effect of taVNS in ZDF rats is associated with changes in the central nervous system (CNS) expression of P2Y1 receptors (P2Y1R). Adult male ZDF rats were subjected to taVNS and transcutaneous non-vagal nerve stimulation (tnVNS). Their food intake and body weight were recorded daily and weekly, respectively. P2Y1R expression in the hypothalamus, amygdala, and hippocampus was evaluated by western blotting. Hypothalamic P2Y1R expressing cells were detected using immunohistochemistry. Naïve ZDF rats were much heavier (p < 0.05) than their lean littermates (ZL rats), with elevated hypothalamic P2Y1R expression (p < 0.05). Further, taVNS but not tnVNS attenuated weight gain (p < 0.05) without decreasing food intake (p > 0.05) and suppressed hypothalamic P2Y1R expression in ZDF rats (p < 0.05). Moreover, P2Y1R showed major expression in astrocytes of ZDF rats' hypothalamus. ZDF rats innately develop obesity associated with elevated hypothalamic P2Y1R expression. taVNS attenuates weight gain in ZDF rats without changes in food intake, suggesting increased energy expenditure. Whether the reduced hypothalamic P2Y1R expression in response to taVNS is mechanistically linked to the increased energy expenditure remains to be determined.
基金：
The author(s) disclosed receipt of the following financial support for the research, authorship,
and/or publication of this article: This work was supported by the Joint Sino-German Research Project [GZ1236], National Natural Science Foundation of China [81674072],
Beijing Municipal Science and Technology Commission [Z161100002616003], China
Scholarship Council [CSC No. 201709920086], Deutscher Akademischer Austauschdienst
[91658555], Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar
Funded Scheme [2016], Beijing Municipal Administration of Hospitals [ZYLX201812],
Beijing Administration of Traditional Chinese Medicine ([2019] No.106), Clinical
Cooperation Ability Construction Project of Chinese and Western Medicine for Major and
Difficult Diseases (Department of Medical Administration, National Administration of
Traditional Chinese Medicine [2018] No.3), National Administration of Traditional Chinese
Medicine (2019XZZX-JB004), and Youth Project of Beijing Shijitan Hospital (2019-q04).
第一作者机构：[1]Acupuncture Department, Beijing Shijitan Hospital, Capital Medical University, Beijing, China[2]Ninth School of Clinical Medicine, Peking University, Beijing, China[3]Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, China[*1]Acupuncture Department, Beijing Shijitan Hospital, Capital Medical University, No. 10 Tieyi Road, Haidian, Beijing 100038, China.
通讯作者：
通讯机构：[1]Acupuncture Department, Beijing Shijitan Hospital, Capital Medical University, Beijing, China[2]Ninth School of Clinical Medicine, Peking University, Beijing, China[3]Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, China[*1]Acupuncture Department, Beijing Shijitan Hospital, Capital Medical University, No. 10 Tieyi Road, Haidian, Beijing 100038, China.[*2]Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, No. 16 Dongzhimen South Street, Dongcheng, Beijing 100700, China
推荐引用方式(GB/T 7714)：
Yu Yutian,He Xun,Zhang Jinling,et al.Transcutaneous auricular vagal nerve stimulation inhibits hypothalamic P2Y1R expression and attenuates weight gain without decreasing food intake in Zucker diabetic fatty rats.[J].SCIENCE PROGRESS.2021,104(2):doi:10.1177/00368504211009669.
APA：
Yu Yutian,He Xun,Zhang Jinling,Tang Chunzhi&Rong Peijing.(2021).Transcutaneous auricular vagal nerve stimulation inhibits hypothalamic P2Y1R expression and attenuates weight gain without decreasing food intake in Zucker diabetic fatty rats..SCIENCE PROGRESS,104,(2)
MLA：
Yu Yutian,et al."Transcutaneous auricular vagal nerve stimulation inhibits hypothalamic P2Y1R expression and attenuates weight gain without decreasing food intake in Zucker diabetic fatty rats.".SCIENCE PROGRESS 104..2(2021)
