机构：[1]The First Clinical Medical College, Guangzhou University of ChineseMedicine, Guangzhou 510405, Guangdong, China[2]Guangzhou University ofChinese Medicine, Guangzhou 510405, Guangdong, China深圳市中医院深圳医学信息中心[3]GuangzhouOrthopedic Hospital, Guangzhou University of Chinese Medicine, Guangzhou510045, Guangdong, China深圳市中医院深圳医学信息中心[4]The Lab of Orthopaedics of Chinese Medicineof Lingnan Medical Research Center, Guangzhou University of ChineseMedicine, Guangzhou 510405, Guangdong, China深圳市中医院深圳医学信息中心[5]Department of JointOrthopaedic, the Third Affiliated Hospital, Guangzhou University of ChineseMedicine, Guangzhou 510405, Guangdong, China[6]Institute of Orthopedics,Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong,China深圳市中医院深圳医学信息中心
Steroid-induced osteonecrosis of the femoral head (ONFH) is a common hip joint disease and is difficult to be diagnosed early. At present, the pathogenesis of steroid-induced ONFH remains unclear, and recognized and effective diagnostic biomarkers are deficient. The present study aimed to identify potentially important genes and signaling pathways involved in steroid-induced ONFH and investigate their molecular mechanisms.
Microarray data sets GSE123568 (peripheral blood) and GSE74089 (cartilage) were obtained from the Gene Expression Omnibus database, including 34 ONFH samples and 14 control samples. Morpheus software and Venn diagram were used to identify DEGs and co-expressed DEGs, respectively. Besides, we conducted Kyoto Encyclopedia of Genome (KEGG) and gene ontology (GO) pathway enrichment analysis. We construct a protein-protein interaction (PPI) network through GEO2R and used cytoHubba to divide the PPI network into multiple sub-networks. Additionally, quantitative real-time polymerase chain reaction (qRT-PCR) was performed to verify the bioinformatics analysis results.
A total of 118 intersecting DEGs were obtained between the peripheral blood and cartilage samples, including 40 upregulated genes and 78 downregulated genes. Then, GO and KEGG pathway enrichment analysis revealed that upregulated DEGs focused on the signaling pathways related to staphylococcus aureus infection, leishmaniasis, antigen processing, and presentation, as well as asthma and graft-versus-host disease. Downregulated genes were concentrated in the FoxO signaling pathway, AMPK signaling pathway, signaling pathway regulating stem cell pluripotency, and mTOR signaling pathway. Some hub genes with high interactions such as CXCR1, FPR1, MAPK1, FOXO3, FPR2, CXCR2, and TYROBP were identified in the PPI network. The results of qRT-PCR demonstrated that CXCR1, FPR1, and TYROBP were upregulated while MAPK1 was downregulated in peripheral blood of steroid-induced ONFH patients. This was consistent with the bioinformatics analysis.
The present study would provide novel insight into the genes and associated pathways involved in steroid-induced ONFH. CXCR1, FPR1, TYROBP, and MAPK1 may be used as potential drug targets and biomarkers for the diagnosis and prognosis of steroid-induced ONFH.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|4 区医学
小类|4 区骨科
最新[2025]版：
大类|3 区医学
小类|3 区骨科
第一作者：
第一作者机构：[1]The First Clinical Medical College, Guangzhou University of ChineseMedicine, Guangzhou 510405, Guangdong, China[2]Guangzhou University ofChinese Medicine, Guangzhou 510405, Guangdong, China[4]The Lab of Orthopaedics of Chinese Medicineof Lingnan Medical Research Center, Guangzhou University of ChineseMedicine, Guangzhou 510405, Guangdong, China[5]Department of JointOrthopaedic, the Third Affiliated Hospital, Guangzhou University of ChineseMedicine, Guangzhou 510405, Guangdong, China[6]Institute of Orthopedics,Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong,China
通讯作者：
通讯机构：[5]Department of JointOrthopaedic, the Third Affiliated Hospital, Guangzhou University of ChineseMedicine, Guangzhou 510405, Guangdong, China[6]Institute of Orthopedics,Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong,China
推荐引用方式(GB/T 7714)：
Tianye Lin,Weijian Chen,Peng Yang,et al.Bioinformatics analysis and identification of genes and molecular pathways in steroid-induced osteonecrosis of the femoral head.[J].Journal of orthopaedic surgery and research.2021,16(1):327.doi:10.1186/s13018-021-02464-9.
APA：
Tianye Lin,Weijian Chen,Peng Yang,Ziqi Li,Qiushi Wei...&Qingwen Zhang.(2021).Bioinformatics analysis and identification of genes and molecular pathways in steroid-induced osteonecrosis of the femoral head..Journal of orthopaedic surgery and research,16,(1)
MLA：
Tianye Lin,et al."Bioinformatics analysis and identification of genes and molecular pathways in steroid-induced osteonecrosis of the femoral head.".Journal of orthopaedic surgery and research 16..1(2021):327
