机构：[1]Institute of Genomic Medicine, College of Pharmacy, Jinan University, Guangzhou 510632,China[2]Department of Anatomy, School of Basic Medical Sciences, Southern Medical University,Guangzhou 510515, China[3]Department of Stomatology, Guangdong Second Traditional Chinese MedicineHospital, Guangzhou 510095, China[4]Department of Microbiology and Immunology, College of Basic Medicineand Public Hygiene, Jinan University, Guangzhou 510632, China
Long-term studies have shown that virus infection affects the energy metabolism of host cells, which mainly affects the function of mitochondria and leads to the hydrolysis of ATP in host cells, but it is not clear how virus infection participates in mitochondrial energy metabolism in host cells. In our study, HUVEC cells were infected with HSV-1, and the differentially expressed genes were obtained by microarray analysis and data analysis. The viral gene encoding protein UL16 was identified to interact with host protein ANT2 by immunoprecipitation and mass spectrometry. We also reported that UL16 transfection promoted oxidative phosphorylation of glucose and significantly increased intracellular ATP content. Furthermore, UL16 was transfected into the HUVEC cell model with mitochondrial dysfunction induced by D-Gal, and it was found that UL16 could restore the mitochondrial function of cells. It was first discovered that viral protein UL16 could enhance mitochondrial function in mammalian cells by promoting mitochondrial metabolism. This study provides a theoretical basis for the prevention and treatment of mitochondrial dysfunction or the pathological process related to mitochondrial dysfunction.
基金：
National Key R&D Program of China (2017YFC1103400), and National Natural
Science Foundation of China (NSFC NO.82074133, 82073747), and Su Yuan biotechnology co. LTD.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|3 区综合性期刊
小类|3 区综合性期刊
最新[2025]版：
大类|3 区综合性期刊
小类|3 区综合性期刊
第一作者：
第一作者机构：[1]Institute of Genomic Medicine, College of Pharmacy, Jinan University, Guangzhou 510632,China[2]Department of Anatomy, School of Basic Medical Sciences, Southern Medical University,Guangzhou 510515, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Li Shiyu,Liu Shuting,Dai Zhenning,et al.The UL16 protein of HSV-1 promotes the metabolism of cell mitochondria by binding to ANT2 protein.[J].Scientific reports.2021,11(1):14001.doi:10.1038/s41598-021-93430-2.
APA：
Li Shiyu,Liu Shuting,Dai Zhenning,Zhang Qian,Xu Yichao...&Sun Hanxiao.(2021).The UL16 protein of HSV-1 promotes the metabolism of cell mitochondria by binding to ANT2 protein..Scientific reports,11,(1)
MLA：
Li Shiyu,et al."The UL16 protein of HSV-1 promotes the metabolism of cell mitochondria by binding to ANT2 protein.".Scientific reports 11..1(2021):14001
