机构：[1]Shenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, China,深圳市中医院深圳医学信息中心[2]Fifth Clinical Medical School, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China,[3]Guangdong Second Traditional Chinese Medicine Hospital (Guangdong Province Engineering Technology Research Institute of T. C. M), Guangzhou, Guangdong, China,[4]Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine, Guangzhou, Guangdong, China,[5]Shenzhen People’s Hospital, Shenzhen, Guangdong, China,深圳市人民医院深圳医学信息中心[6]Guangdong Yifang Pharmaceutical Co., Ltd. Foshan, Guangdong, China,[7]College of Chinese Traditional Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China,[8]Yangchun Field Test and Demonstration of Amomum Villosum, Yangchun, China
To explore the influence of biological characteristics on the yield of Amomum villosum Lour. and Amomum longiligulare T. L. Wu, to find an effective pollen viability evaluation method and storage method to solve the problem of the low yield of Amomum plants.Five germplasm of Amomum plants were used to investigate the effects of the phenological phase, pollen viability, and stigma receptivity on natural and artificial fruit set.Amomum longiligulare T. L. Wu showed late flowering, and its natural pollination rate is higher than that of Amomum villosum Lour. In all germplasm, the artificial pollination rate and fruit setting rate are more than 3 times higher than that under natural conditions. Fruits begin to drop seven days after successful pollination, and the fruit drop is basically stable after one month. The hybridization verification showed that TTC method was simpler and more accurate than in vitro germination method. Optimal storage conditions for pollen are 4°C and high humidity. After 36 h of storage, pollen can still be used for artificial cross-pollination or as hybrid parents.The special biological characteristics are the fundamental reason for the low natural pollination rate of Amomum plants. The accurate measurement method of Amomum plants pollen is the TTC method, and storage at 4°C and high humidity can increase the yield, which was six times that of the natural yield.
基金：
National Key R&D Program of China
(2017YFC1701102), Key-Area Research and
Development Program of Guangdong Province
(No.2020B20221002), Project of Administration of
Traditional Chinese Medicine of Guangdong
Province of China(20191281), and National Major Scientific and Technological Special Project for
“Significant New Drugs Development”:
(2018ZX09721005-002-004)
第一作者机构：[1]Shenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, China,[2]Fifth Clinical Medical School, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China,[3]Guangdong Second Traditional Chinese Medicine Hospital (Guangdong Province Engineering Technology Research Institute of T. C. M), Guangzhou, Guangdong, China,[4]Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine, Guangzhou, Guangdong, China,
通讯作者：
推荐引用方式(GB/T 7714)：
Yang Ruipei,Wang Jie,Gao Wei,et al.Research on the reproductive biological characteristics of Amomum villosum Lour. and Amomum longiligulare T. L. Wu.[J].PLOS ONE.2021,16(8):doi:10.1371/journal.pone.0250335.
APA：
Yang Ruipei,Wang Jie,Gao Wei,Jiang Ye,Su Jing...&He Guozhen.(2021).Research on the reproductive biological characteristics of Amomum villosum Lour. and Amomum longiligulare T. L. Wu..PLOS ONE,16,(8)
MLA：
Yang Ruipei,et al."Research on the reproductive biological characteristics of Amomum villosum Lour. and Amomum longiligulare T. L. Wu.".PLOS ONE 16..8(2021)
