详情页 - 广东省中医院知识库

当前位置：首页 > 详情页

Dopaminergic signaling in prefrontal cortex contributes to the antidepressant effect of electroacupuncture: An iTRAQ-based proteomics analysis in a rat model of CUMS.

文献详情

资源类型：

Pubmed体系：

作者：

机构： [1]School of Chinese Medicine, The University of Hong Kong, Pok Fu Lam, Hong Kong [2]School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China [3]Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China

出处：

DOI：

ISSN：

关键词： chronic unpredictable mild stress (CUMS) depression dopaminergic signaling pathway electroacupuncture iTRAQ prefrontal cortex

摘要：

Electroacupuncture (EA) is used as an adjunctive treatment for depression. This study was conducted to evaluate the efficacy and mechanisms of EA in the depressive rat model induced by chronic unpredictable mild stress (CUMS) in male adult Wistar rats. The underlying mechanisms were explored by using isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analysis of the proteins in the prefrontal cortex (PFC), and observing the number of the PFC neurons stained with hematoxylin and eosin (H&E) and synaptic morphological changes under transmission electron microscopy (TEM). The results showed that EA plus paroxetine (EA + Par) for 1 week significantly relieved depression-like anhedonia symptoms and improved anxiety-like behavior, accompanied by the improvements in synaptic morphology and a significant increase of PFC neurons. Moreover, EA or paroxetine alone significantly alleviated anhedonia symptoms after 2 weeks of intervention. Additionally, iTRAQ analysis showed that dopaminergic signaling was significantly altered in CUMS rats after 1 week of EA treatment. As the critical enzyme of this pathway, aromatic-l-amino-acid decarboxylase (DDC) was significantly upregulated after the treatment with EA + Par for 1 week. These findings suggested that the dopaminergic signaling pathway in PFC may be involved in the antidepressant mechanisms of EA.© 2021 American Association for Anatomy.

基金：

语种：

PubmedID：

中科院(CAS)分区：

出版当年[2020]版：

大类 | 4 区医学

小类 | 4 区解剖学与形态学

最新[2025]版：

大类 | 4 区医学

小类 | 3 区解剖学与形态学

第一作者：

第一作者机构： [1]School of Chinese Medicine, The University of Hong Kong, Pok Fu Lam, Hong Kong

共同第一作者：

通讯作者：

通讯机构： [2]School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China [*1]No. 1023-1063, Shatai North Road, Baiyun District, Guangzhou, Guangdong Province, 510515, China

推荐引用方式(GB/T 7714)：

APA：

MLA：

相关文献

[1]Mechanism Underlying Acupuncture-Ameliorated Depressive Behaviors by Enhancing Glial Glutamate Transporter in Chronic Unpredictable Mild Stress (CUMS) Rats [2]PGAM5 knockout causes depressive-like behaviors in mice via ATP deficiency in the prefrontal cortex [3]Xiaoyaosan promotes neurotransmitter transmission and alleviates CUMS-induced depression by regulating the expression of Oct1 and Oct3 in astrocytes of the prefrontal cortex [4]The rapid antidepressant effect of acupuncture on two animal models of depression by inhibiting M1-Ach receptors regulates synaptic plasticity in the prefrontal cortex [5]Early Life Stress Induces Different Behaviors in Adolescence and Adulthood May Related With Abnormal Medial Prefrontal Cortex Excitation/Inhibition Balance. [6]Neural circuitry among connecting the hippocampus, prefrontal cortex and basolateral amygdala in a mouse depression model: Associations correlations between BDNF levels and BOLD - fMRI signals. [7]Teasaponin improves leptin sensitivity in the prefrontal cortex of obese mice. [8]eEF2 in the prefrontal cortex promotes excitatory synaptic transmission and social novelty behavior [9]The Effects of Transcranial Direct Current Stimulation Versus Electroacupuncture on Working Memory in Healthy Subjects [10]Electroacupuncture Improves Antidepressant Effects in CUMS Rats by Protecting Hippocampal Synapse and Mitochondrion: An Ultrastructural and iTRAQ Proteomic Study.