机构：[1]Department of Otorhinolaryngology Head and Neck Surgery, Shenzhen Fuyong People's Hospital, Shenzhen, Guangdong, 518103, China.深圳市康宁医院深圳医学信息中心[2]Department of Orthopaedics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 430071, China.[3]Shenzhen Baoan Authentic TCM Therapy Hospital, Shenzhen, Guangdong, 518101, China.[4]Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan, Hubei, 442008, China.[5]Yangtze University, Health Science Center, Jingzhou, Hubei, 434025, China
An electrochemical aptasensor with high sensitivity, specificity, and good intra-day reproducibility is reported to meet the detection needs of vascular endothelial growth factor (VEGF). The toehold-mediated strand displacement recycling amplification and VEGF aptamer are integrated in the biosensor. The probe A is hybridized with the VEGF aptamer to form the probe A-aptamer complex. When VEGF is introduced, the aptamer specifically binds with VEGF, and probe A can be liberated. Then, the free probe A captures the toehold region of the Hp1, leading the exposure of the toehold region on the other end of Hp1. Similarly, Hp2 and Hp3 are also immobilized on the surface of the electrode; thus, the methylene blue labelled on Hp2 and Hp3 causes the current response. With the signal transduction mechanism, the expression level of VEGF can be detected quantitatively. With a series of optimizations of sensor parameters, high sensitivity and specificity of the VEGF detection sensor can be achieved with a detection limit as low as 10 pg mL-1. This significant performance has good intra-day reproducibility, and it can be applied to human biological samples such as serum, urine, and saliva to detect the VEGF content.
基金：
National Natural Science
Foundation of China (81872509), Bao'an TCM Development
Foundation (2020KJCX-KTYJ-200), the Internal Research Project
of Shenzhen Baoan Authentic TCM Therapy Hospital
(BCZY2021003 and BCZY2021007), Baoan District Medical and
Health Basic Research Project (2020JD491), Chinese Medicine
Research Fund of Health Commission of Hubei Province
(ZY2021M038 and ZY2021M051), Hubei Province Health and
Family Planning Scientic Research Project (WJ2021F052,
WJ2021M062 and WJ2021M063), the Science and Technology
Key Program of Shiyan (No. 21Y77) and the Science and Technology
Planning Project of Shenzhen Municipality
(JCYJ20210305163906017).
第一作者机构：[1]Department of Otorhinolaryngology Head and Neck Surgery, Shenzhen Fuyong People's Hospital, Shenzhen, Guangdong, 518103, China.
共同第一作者：
通讯作者：
通讯机构：[2]Department of Orthopaedics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 430071, China.[3]Shenzhen Baoan Authentic TCM Therapy Hospital, Shenzhen, Guangdong, 518101, China.[4]Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan, Hubei, 442008, China.[5]Yangtze University, Health Science Center, Jingzhou, Hubei, 434025, China
推荐引用方式(GB/T 7714)：
Gao Zhong,Ren Fangling,Yang Guangyi,et al.A highly sensitive electrochemical aptasensor for vascular endothelial growth factor detection based on toehold-mediated strand displacement reaction.[J].ANALYTICAL METHODS.2021,13(41):4934-4940.doi:10.1039/d1ay01263k.
APA：
Gao Zhong,Ren Fangling,Yang Guangyi,Feng Guangjun,Wu Lun...&Chen Qinhua.(2021).A highly sensitive electrochemical aptasensor for vascular endothelial growth factor detection based on toehold-mediated strand displacement reaction..ANALYTICAL METHODS,13,(41)
MLA：
Gao Zhong,et al."A highly sensitive electrochemical aptasensor for vascular endothelial growth factor detection based on toehold-mediated strand displacement reaction.".ANALYTICAL METHODS 13..41(2021):4934-4940
