机构：[1]Department of Pathology, Ganzhou People's Hospital, Ganzhou, 341000, China.[2]College of Pharmacy, Gannan Medical University, Ganzhou, 341000, China.[3]Department of Orthopedics, the First Affiliated Hospital of Gannan Medical University, Ganzhou, 341000, China.[4]Key Laboratory of Traditional Chinese Medicine & New Pharmacy Development, Guangdong Medical University, Dongguan, 523808, China.
Dyslipidemia has been associated with the development of osteoarthritis. Our previous study found that 5,7,3',4'-tetramethoxyflavone (TMF) exhibited protective activities against the pathological changes of osteoarthritis. Aim: To investigate the roles of TMF in regulating ABCA1-mediated cholesterol metabolism. Methods: Knockdown and overexpression were employed to study gene functions. Protein-protein interaction was investigated by co-immunoprecipitation, and the subcellular locations of proteins were studied by immunofluorescence. Results: IL-1β decreased ABCA1 expression and induced apoptosis. Therapeutically, TMF ameliorated the effects of IL-1β. FOXO3a knockdown expression abrogated the effects of TMF, and FOXO3a overexpression increased ABCA1 expression by interacting with LXRα. TMF promoted FOXO3a nuclear translocation by activating SIRT1 expression. Conclusions: TMF ameliorates cholesterol dysregulation by increasing the expression of FOXO3a/LXRα/ABCA1 signaling through SIRT1 in C28/I2 cells.
基金：
National Science Foundation of China (82060407 and 81860388), the Natural Science
Foundation of Jiangxi Province (20202BABL206122 and 20202BABL206134), Scientific Research Fund of Jiangxi Provincial Education
Department (GJJ201501 and GJJ201510) and Innovative Teamwork Project of Gannan Medical University (TD201707).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|2 区医学
小类|3 区药物化学
最新[2025]版：
大类|4 区医学
小类|4 区药物化学
第一作者：
第一作者机构：[1]Department of Pathology, Ganzhou People's Hospital, Ganzhou, 341000, China.
通讯作者：
推荐引用方式(GB/T 7714)：
Peng Fang,Huang Xianhua,Shi Weimei,et al.5,7,3',4'-tetramethoxyflavone ameliorates cholesterol dysregulation by mediating SIRT1/FOXO3a/ABCA1 signaling in osteoarthritis chondrocytes.[J].Future medicinal chemistry.2021,13(24):2153-2166.doi:10.4155/fmc-2021-0247.
APA：
Peng Fang,Huang Xianhua,Shi Weimei,Xiao Yaosheng,Jin Qi...&Wu Longhuo.(2021).5,7,3',4'-tetramethoxyflavone ameliorates cholesterol dysregulation by mediating SIRT1/FOXO3a/ABCA1 signaling in osteoarthritis chondrocytes..Future medicinal chemistry,13,(24)
MLA：
Peng Fang,et al."5,7,3',4'-tetramethoxyflavone ameliorates cholesterol dysregulation by mediating SIRT1/FOXO3a/ABCA1 signaling in osteoarthritis chondrocytes.".Future medicinal chemistry 13..24(2021):2153-2166
