机构：[1]RayBiotech Life, 3607 Parkway Lane, Peachtree Corners, GA 30092, USA.[2]RayBiotech Guangzhou, 79 Ruihe Road, Huangpu district, Guangzhou 510630, Guangdong, China.[3]Department of Laboratory Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510000, Guangdong, China.[4]South China Biochip Research Center, 79 Ruihe Road, Huangpu District, Guangzhou 510600, Guangdong, China.[5]Affiliated Cancer Hospital and Institute of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510095, China.[6]Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510120, China.广东省中医院[7]Present address: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Krone Engineering Biosystems Building, 950 Atlantic Dr. NW, Atlanta, GA 30332, USA.
Dried blood samples (DBSs) have many advantages; yet, impediments have limited the clinical utilization of DBSs. We developed a novel volumetric sampling device that collects a precise volume of blood, which overcomes the heterogeneity and hematocrit issues commonly encountered in a traditional DBS card collection as well as allowing for more efficient extraction and processing procedures and thus, more efficient quantitation, by using the entire sample. We also provided a thorough procedure validation using this volumetric DBS collection device with an established quantitative proteomics analysis method, and then analyzed 1000 proteins using this approach in DBSs concomitantly with serum for future consideration of utility in clinical applications. Our data provide a first step in the establishment of a DBS database for the broad application of this sample type for widespread use in clinical proteomic and other analyses applications.
基金：
We would like to express our thanks for the support of RayBiotech innovative research fund, Guangzhou Innovation
Leadership Team (CXLJTD-201602), Guangdong Province Key Technologies R&D Program for "Precision
Medicine and Stem Cells" (2019B020231002, 2019B020227004), National Science and Technology Major Project
of China (No. 2018ZX10732-401-003-012), the Industry-University-Research Collaborative Innovation Special
Project of Guangzhou (201802030001). Guangzhou Basic and Applied Basic Research Project (202002030242).
Basic Research Plan of Guangzhou People’s Livelihood Science and Technology Project (202002020021).
第一作者机构：[1]RayBiotech Life, 3607 Parkway Lane, Peachtree Corners, GA 30092, USA.
共同第一作者：
通讯作者：
通讯机构：[1]RayBiotech Life, 3607 Parkway Lane, Peachtree Corners, GA 30092, USA.[2]RayBiotech Guangzhou, 79 Ruihe Road, Huangpu district, Guangzhou 510630, Guangdong, China.[4]South China Biochip Research Center, 79 Ruihe Road, Huangpu District, Guangzhou 510600, Guangdong, China.[5]Affiliated Cancer Hospital and Institute of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510095, China.[6]Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510120, China.
推荐引用方式(GB/T 7714)：
Kelly Whittaker,Ying‑Qing Mao,Yongping Lin,et al.Dried blood sample analysis by antibody array across the total testing process[J].SCIENTIFIC REPORTS.2021,11(1):doi:10.1038/s41598-021-99911-8.
APA：
Kelly Whittaker,Ying‑Qing Mao,Yongping Lin,Huihua Zhang,Siwei Zhu...&Ruo‑Pan Huang.(2021).Dried blood sample analysis by antibody array across the total testing process.SCIENTIFIC REPORTS,11,(1)
MLA：
Kelly Whittaker,et al."Dried blood sample analysis by antibody array across the total testing process".SCIENTIFIC REPORTS 11..1(2021)
