机构：[a]Institute of Bioengineering, Guangdong Academy of Sciences, Guangzhou, People’s Republic of China[b]Key Laboratory of Quality Evaluation of Chinese Medicine of the Guangdong Provincial Medical Products Administration, the Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, People’s Republic of China广东省中医院[c]Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, People’s Republic of China
Coriolopsis trogii is a typical thermotolerant basidiomycete fungus, but its thermotolerance mechanisms are currently unknown. In this study, two monokaryons of C. trogii strain Ct001 were assembled: Ct001_29 had a genome assembly size of 38.85Mb and encoded 13,113 genes, while Ct001_31 was 40.19 Mb in length and encoded 13,309 genes. Comparative intra- and interstrain genomic analysis revealed the rich genetic diversity of C. trogii, which included more than 315,194 singlenucleotide polymorphisms (SNPs), 30,387 insertion/deletions (indels), and 1,460 structural variations. Gene family analysis showed that the expanded families of C. trogii were functionally enriched in lignocellulose degradation activities. Furthermore, a total of 14 allelic pairs of heat shock protein 20 (HSP20) genes were identified in the C. trogii genome. The expression profile obtained from RNA sequencing (RNA-Seq) showed that four tandem-duplicated allelic pairs, HSP20.5 to HSP20.8, had more than 5-fold higher expression at 35 degrees C than at 25 degrees C. In particular, HSP20.5 and HSP20.8 were the most highly expressed HSP20 genes. Allelic expression bias was found for HSP20.5 and HSP20.8; the expression of Ct29HSP20.8 was at least 1.34-fold higher than that of Ct31HSP20.8, and that of Ct31HSP20.5 was at least 1.5-fold higher than that of Ct29HSP20.5. The unique structural and expression profiles of the HSP20 genes revealed by these haplotyperesolved genomes provide insight into the molecular mechanisms of high-temperature adaptation in C. trogii. IMPORTANCE Heat stress is one of the most frequently encountered environmental stresses for most mushroom-forming fungi. Currently available fungal genomes are mostly haploid because high heterozygosity hinders diploid genome assembly. Here, two haplotype genomes of C. trogii, a thermotolerant basidiomycete, were assembled separately. A conserved tandem cluster of four HSP20 genes showing allele-specific expression was found to be closely related to high-temperature adaptation in C. trogii. The obtained haploid genomes and their comparison offer a more thorough understanding of the genetic background of C. trogii. In addition, the responses of HSP20 genes at 35 degrees C, which may contribute to the growth and survival of C. trogii at high temperatures, could inform the selection and breeding of elite strains in the future.
基金：
This research was funded by the GDAS' Project of Science and Technology
Development (grant 2020GDASYL-20200103071), by the “13th Five-Year Plan” Key Field
Research Project of the China Academy of Chinese Medical Sciences (grant ZZ10-007), by
the Project Quality Standard System Construction for the Whole Industry Chain of Chinese
Medicinal Decoction Pieces from Guangdong Provincial Drug Administration of China
(grant 002009/2019KT1261/2020ZDB25), and by the Special Foundation of Guangzhou Key
Laboratory (grant 202002010004).
第一作者机构：[a]Institute of Bioengineering, Guangdong Academy of Sciences, Guangzhou, People’s Republic of China
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通讯作者：
推荐引用方式(GB/T 7714)：
Lining Wang,Baosheng Liao,Lu Gong,et al.Haploid Genome Analysis Reveals a Tandem Cluster of Four HSP20 Genes Involved in the High-Temperature Adaptation of Coriolopsis trogii[J].MICROBIOLOGY SPECTRUM.2021,9(1):doi:10.1128/Spectrum.00287-21.
APA：
Lining Wang,Baosheng Liao,Lu Gong,Shuiming Xiao&Zhihai Huang.(2021).Haploid Genome Analysis Reveals a Tandem Cluster of Four HSP20 Genes Involved in the High-Temperature Adaptation of Coriolopsis trogii.MICROBIOLOGY SPECTRUM,9,(1)
MLA：
Lining Wang,et al."Haploid Genome Analysis Reveals a Tandem Cluster of Four HSP20 Genes Involved in the High-Temperature Adaptation of Coriolopsis trogii".MICROBIOLOGY SPECTRUM 9..1(2021)
