机构：[1]MOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou, China[2]The First Affiliated Hospital, Jinan University, Guangzhou, China[3]Gastrointestinal Surgery, The First People’s Hospital of FoShan, Foshan, China[4]Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Guangdong Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China[5]Research Laboratory of Zhuang & Yao Medicine, Guangxi International Zhuang Medicine Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning, Guangxi, China
Lung adenocarcinoma (LAC) is one of the most common pulmonary adenocarcinomas with a high peak of mortality, and metastasis is the main culprit of LAC deaths. microRNAs play important role in cancer metastasis, and thus are regarded as potential diagnostic and prognostic markers for human cancers. However, many miRNAs exhibit dual roles in diverse cellular contexts. Here, we revealed that hsa-miR-335, a previously reported tumor suppressor, exhibited an oncogenic role in LAC. Overexpression of miR-335 enhanced the abilities of A549 and H1299 cells to invade and migrate by regulating epithelial-mesenchymal transition, while inhibition of miR-335 exhibited an opposite effect in vitro and in vivo. Mechanically, miR-335 inhibited the expression of Copine-1 (CPNE1), an NF-kappa B suppressor, through interacting with its mRNA 3 ' UTR, while mutating the binding sites abolished this inhibitory effect. This finding not only highlights the suppressive effect of CPNE1 on cell motility, but also provides new insight into miR-335 in promoting LAC metastasis.
基金：
NationalNatural Science Foundation of
China, Grant/Award Numbers: 82002948,
82103208;GuangdongNatural Science
Research Grant,Grant/Award Number:
2019A1515110597;National Innovation and
EntrepreneurshipTraining ProgramFor
Undergraduate, Grant/Award Number:
202110559085
第一作者机构：[1]MOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou, China[*1]Institute of Life and Health Engineering, College of Life Science andTechnology, Jinan University, Guangzhou 510632,China
共同第一作者：
通讯作者：
通讯机构：[1]MOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou, China[2]The First Affiliated Hospital, Jinan University, Guangzhou, China[*1]Institute of Life and Health Engineering, College of Life Science andTechnology, Jinan University, Guangzhou 510632,China
推荐引用方式(GB/T 7714)：
YangWang,Jing Zhang,Li-Ye Zhong,et al.Hsa-miR-335 enhances cell migration and invasion in lung adenocarcinoma through targeting Copine-1[J].MEDCOMM.2021,2(4):810-820.doi:10.1002/mco2.93.
APA：
YangWang,Jing Zhang,Li-Ye Zhong,Shang-Jia Huang,Nan-Nan Yu...&Qing-Yu He.(2021).Hsa-miR-335 enhances cell migration and invasion in lung adenocarcinoma through targeting Copine-1.MEDCOMM,2,(4)
MLA：
YangWang,et al."Hsa-miR-335 enhances cell migration and invasion in lung adenocarcinoma through targeting Copine-1".MEDCOMM 2..4(2021):810-820
医学