4D-quantitative proteomics signature of asthenozoospermia and identification of extracellular matrix protein 1 as a novel biomarker for sperm motility.
机构：[a]Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen, 518036, P. R. China.北京大学深圳医院深圳医学信息中心香港大学深圳医院[b]Reproductive Medicine Center, Peking University Shenzhen Hospital, P. R. China北京大学深圳医院深圳医学信息中心[c]Medical Laboratory Department, Shenzhen Hospital (Futian) of Guangzhou University of Chinese Medicine, P. R. China广州中医药大学深圳医院深圳市康宁医院深圳医学信息中心
Asthenozoospermia (AZS), diagnosed by reduced sperm motility, is one of the major causes of male infertility. However, AZS has no effective therapeutic treatment and the underlying molecular mechanism remains largely unclear. In this study, state-of-the-art 4D-quantitative proteomics analysis was used to compare the protein profiling between 7 normozoospermic and 11 asthenozoospermic sperm samples. Overall, 4718 proteins were identified and 1430 differential abundant proteins were found in the two groups. The differentially expressed proteins were analyzed by GO and KEGG. The core deregulated proteins and pathways associated sperm motility dysfunction included energy metabolism and the sperm structure. Integrative analysis further identified extracellular matrix protein 1 (ECM1) as a novel biomarker related to AZS. Our study could provide new insights into the molecular basis of low sperm motility. The mass spectrometric data are available via ProteomeXchange with identifier PXD027637.
基金：
This work was supported by grants from the Shenzhen Project
of Science and Technology (KQJSCX20180327124222443) and
the Guangdong Natural Science Foundation (2018A030313669).
第一作者机构：[a]Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen, 518036, P. R. China.
通讯作者：
推荐引用方式(GB/T 7714)：
Jie Yang,Qunlong Liu,Bohai Yu,et al.4D-quantitative proteomics signature of asthenozoospermia and identification of extracellular matrix protein 1 as a novel biomarker for sperm motility.[J].MOLECULAR OMICS.2022,18(1):83-91.doi:10.1039/d1mo00257k.
APA：
Jie Yang,Qunlong Liu,Bohai Yu,Baoquan Han&Bo Yang.(2022).4D-quantitative proteomics signature of asthenozoospermia and identification of extracellular matrix protein 1 as a novel biomarker for sperm motility..MOLECULAR OMICS,18,(1)
MLA：
Jie Yang,et al."4D-quantitative proteomics signature of asthenozoospermia and identification of extracellular matrix protein 1 as a novel biomarker for sperm motility.".MOLECULAR OMICS 18..1(2022):83-91
