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MiRNA-320a-5p contributes to the homeostasis of osteogenesis and adipogenesis in bone marrow mesenchymal stem cell.

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机构： [1]Medical Center of Hip, Luoyang Orthopedic-Traumatological Hospital (Orthopedics Hospital of Henan Province), Luoyang, Henan, 471002, PR China. [2]Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong, 510405, PR China. [3]Hunan University of Traditional Chinese Medicine, Changsha, Hunan, 410208, PR China. [4]Institute of Orthopaedics of Guangzhou University of Traditional Chinese Medicine, Guangzhou, 510240, PR China. [5]The Third Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou, 510240, PR China. [6]Henan University of Traditional Chinese Medicine, Zhengzhou, Henan, 450046, PR China.

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A number of miRNAs and their targets were dragged in the differentiation of bone marrow mesenchymal stem cells (BMSCs). We aimed to elaborate the underlying molecular mechanisms of miRNA-320a in the osteoblast and adipocyte differentiation.Trauma-induced osteonecrosis of the femoral head (TIONFH) and normal control samples (n = 10 for each group) were collected, followed by miRNA chip analysis to identify the differentially expressed miRNAs. H&E staining was used to observe the pathological development of TIONFH. Lentiviral vector was used for overexpression and inhibition of miRNA-320a in vitro. Quantitative real-time PCR (qPCR), Western blotting and immunohistochemistry staining were employed to determine the expression of interested genes at mRNA or protein level. Luciferase report assay was employed to determine the binding of miRNA-320a and RUNX2. Alkaline phosphatase (ALP) and Alizarin red staining were performed to observe the osteogenesis and Oil red O staining were conducted to visualize the adipogenesis.Expression of miRNA-320a was up-regulated while RUNX2 expression was down-regulated in TIONFH than Normal control. Luciferase report assay confirmed that miRNA-320a directly targeted to the 3'UTR of RUNX2. miRNA-320a overexpression significantly declined the expressions of osteogenesis-related markers: RUNX2, OSTERIX, Collagen I, Osteocalcin and Osteopontin. ALP and Alizarin red staining confirmed the inhibition function of miRNA-320a in osteogenesis of BMSCs. miRNA-320a inhibition significantly decreased the expression of adipogenesis-related markers: AP2, C/EBPα, FABP4 and PPARγ. Oil Red O staining confirmed the miRNA-320a inhibition reduced adipogenesis of BMSCs.miRNA-320a inhibits osteoblast differentiation via targeting RUNX2 and promotes adipocyte differentiation of BMSCs.© 2022 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.

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出版当年[2021]版：

大类 | 3 区工程技术

小类 | 4 区细胞与组织工程 4 区工程：生物医学

最新[2025]版：

大类 | 3 区医学

小类 | 3 区细胞与组织工程 3 区工程：生物医学

第一作者：

第一作者机构： [1]Medical Center of Hip, Luoyang Orthopedic-Traumatological Hospital (Orthopedics Hospital of Henan Province), Luoyang, Henan, 471002, PR China. [2]Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong, 510405, PR China.

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通讯作者：

通讯机构： [1]Medical Center of Hip, Luoyang Orthopedic-Traumatological Hospital (Orthopedics Hospital of Henan Province), Luoyang, Henan, 471002, PR China. [*1]Medical Center of Hip, Luoyang Orthopedic-Traumatological Hospital (Orthopedics Hospital of Henan Province), No. 82 Qiming South Road, Luoyang, Henan, 471002, PR China

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