详情页 - 广东省中医院知识库

当前位置：首页 > 详情页

NAP1L1 promotes tumor proliferation through HDGF/C-JUN signaling in ovarian cancer.

15| 认领 | 导出 | 链接全文 |

文献详情

资源类型：

WOS体系：

Pubmed体系：

收录情况： ◇ SCIE

作者：

机构： [1]Department of Obstetrics and Gynecology, The Third Affliated Hospital, Southern Medical University, Guangzhou, 510500, Guangdong, China. [2]The Second School of Clinical Medicine, Zhujiang Hospital, Southern Medical University, Southern Medical University, Guangzhou, Guangdong, 510280, China. [3]Cancer Center, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 510315, Guangdong, China. [4]First People's Hospital of Zhaoqing, Zhaoqing, 2102013, Guangdong, China. [5]Department of Obstetrics and Gynecology, The Third Affliated Hospital, Southern Medical University, 183 Zhongshan Dadaoxi West, Guangzhou, 510500, Guangdong, China. guosq2005@126.Com.

出处：

DOI：

ISSN：

摘要：

Nucleosome assembly protein 1-like 1 (NAP1L1) is highly expressed in various types of cancer and plays an important role in carcinogenesis, but its specific role in tumor development and progression remains largely unknown. In this study, we suggest the potential of NAP1L1 as a prognostic biomarker and therapeutic target for the treatment of ovarian cancer (OC).In our study, a tissue microarray (TMA) slide containing specimens from 149 patients with OC and 11 normal ovarian tissues underwent immunohistochemistry (IHC) to analyze the correlation between NAP1L1 expression and clinicopathological features. Loss-of- function experiments were performed by transfecting siRNA and following lentiviral gene transduction into SKOV3 and OVCAR3 cells. Cell proliferation and the cell cycle were assessed by the Cell Counting Kit-8, EDU assay, flow cytometry, colony formation assay, and Western blot analysis. In addition, co-immunoprecipitation (Co-IP) and immunofluorescence assays were performed to confirm the relationship between NAP1L1 and its potential targets in SKOV3/OVCAR3 cells.High expression of NAP1L1 was closely related to poor clinical outcomes in OC patients. After knocking down NAP1L1 by siRNA or shRNA, both SKOV3 and OVCAR3 cells showed inhibition of cell proliferation, blocking of the G1/S phase, and increased apoptosis in vitro. Mechanism analysis indicated that NAP1L1 interacted with hepatoma-derived growth factor (HDGF) and they were co-localized in the cytoplasm. Furthermore, HDGF can interact with jun proto-oncogene (C-JUN), an oncogenic transformation factor that induces the expression of cyclin D1 (CCND1). Overexpressed HDGF in NAP1L1 knockdown OC cells not only increased the expression of C-JUN and CCND1, but it also reversed the suppressive effects of si-NAP1L1 on cell proliferation.Our data demonstrated that NAP1L1 could act as a prognostic biomarker in OC and can interact with HDGF to mediate the proliferation of OC, and this process of triggered proliferation may contribute to the activation of HDGF/C-JUN signaling in OC cells.© 2022. The Author(s).

基金：

语种：

WOS：

PubmedID：

中科院(CAS)分区：

出版当年[2021]版：

大类 | 2 区

医学

小类 | 3 区肿瘤学

最新[2025]版：

大类 | 3 区医学

小类 | 3 区肿瘤学

JCR分区：

出版当年[2020]版：

Q2 ONCOLOGY

最新[2023]版：

Q2 ONCOLOGY

影响因子： 3.4 最新[2023版] 3.8 最新五年平均 4.43 出版当年[2020版] 4.372 出版当年五年平均 3.15 出版前一年[2019版] 4.638 出版后一年[2021版]

第一作者：

第一作者机构： [1]Department of Obstetrics and Gynecology, The Third Affliated Hospital, Southern Medical University, Guangzhou, 510500, Guangdong, China. [2]The Second School of Clinical Medicine, Zhujiang Hospital, Southern Medical University, Southern Medical University, Guangzhou, Guangdong, 510280, China.

通讯作者：

推荐引用方式(GB/T 7714)：

APA：

MLA：