A high-performance liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI-MS') method has been developed to determine ginsenoside Rd in human plasma and to identify its metabolites in rat urine. The plasma and urine samples were pretreated by solid phase extraction (SPE) prior to analyses. In this work, gentiopicroside was used as the internal standard. The lower limit of quantification (LLOQ) for Rd in human plasma was 3 ng/ml. The average half-life time in plasma was detected as 19.29 h, when 10 mg of ginsenoside Rd was administrated intravenously to the volunteers. Seven metabolites including three oxygenated, two combined and two hydrolyzed components were identified in rat urine samples by using LC-MS and MS-MS, when ginsenoside Rd administered either orally or intravenously. (C) 2007 Elsevier B.V. All rights reserved.
语种：
中文
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2006]版：
大类|3 区化学
最新[2025]版：
大类|3 区医学
小类|3 区生化研究方法3 区分析化学
JCR分区：
出版当年[2005]版：
Q1CHEMISTRY, ANALYTICALQ2BIOCHEMICAL RESEARCH METHODS
最新[2023]版：
Q2BIOCHEMICAL RESEARCH METHODSQ2CHEMISTRY, ANALYTICAL
第一作者机构：[1]Guangzhou Univ TCM, Second Affiliated Hosp, Guangzhou 510120, Peoples R China; Jinan Univ, Inst Life, Guangzhou, Peoples R China; Jinan Univ, Inst Hlth Engn, Guangzhou, Peoples R China
通讯作者：
通讯机构：[*1]Guangzhou Univ TCM, Second Affiliated Hosp, Guangzhou 510120, Peoples R China
推荐引用方式(GB/T 7714)：
Yang Liu,Deng Yuanhui,Xu Shunjun,等.In vivo pharmacokinetic and metabolism studies of ginsenoside Rd[J].JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES.2007,854(1-2):77-84.
