Acupuncture has been applied in the clinic to treat visceral pain for a long time. However, the underlying mechanism still remains unknown. In the present study, extrasegmental analgesia of electroacupuncture (EA) at orofacial acupoints on visceral pain rats was investigated. The results revealed that nociceptive EA stimulation applied at heterotopic acupoints or nonacupoints to activate A(delta) and/or C fibers induced c-fos expression in the paratrigeminal nucleus (PTN) and significantly inhibited acetic acid-induced abdominal contractions and c-fos expression in the nucleus of the solitary tract (NTS). However, non-nociceptive EA or non-EA stimulation applied at heterotopic acupoints was totally ineffective. After infraorbital nerves transaction or pretreated by capsaicin, the EA analgesia was dramatically inhibited. Snake venom pretreatment had no influence on this analgesia. Consequently, heterotopic EA stimulation trigger the pain-inhibiting effect of diffuse noxious inhibitory controls (DNIC), in which PTN-NTS secondary neural pathway may be involved and small-diameter (A(delta) and/or C) fibers are crucial. (c) 2010 Elsevier B.V. All rights reserved.