Identification of new dianthrone glycosides from Polygonum multiflorum Thunb. using high-performance liquid chromatography coupled with LTQ-Orbitrap mass spectrometry detection: a strategy for the rapid detection of new low abundant metabolites from traditional Chinese medicines
A simple, rapid method was developed using on-line high-performance liquid chromatography (HPLC)/electrospray ionization (ESI)-linear ion trap-Orbitrap hybrid mass spectrometry (LTQ-Orbitrap) to analyze bioactive constituents in the extract of Polygonum multiflorum Thunb., a traditional Chinese medicine (TCM). The qualitative analysis was carried out both in the negative and positive mode to acquire accurate mass data in full scan and data dependent MSn scan. Twenty eight new dianthrone glycosides were characterized and their fragmentation behaviors were first exploited by our LC-HR-MSn method. The identification and structure analysis of these chemicals provided essential data for further phytochemical studies of P. multiflorum. Moreover, the results of the present investigations indicated the relevance and usefulness of the combination of HPLC and LTQ-Orbitrap MS to detect and identify new natural products.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81073052]
第一作者机构：[1] Guangzhou Univ Chinese Med, Coll Clin Med 2, Lab Chinese Mat Med Preparat, Guangdong Prov Inst TCM, Guangzhou, Guangdong, Peoples R China
通讯作者：
通讯机构：[1] Guangzhou Univ Chinese Med, Coll Clin Med 2, Lab Chinese Mat Med Preparat, Guangdong Prov Inst TCM, Guangzhou, Guangdong, Peoples R China[*1]Guangzhou Univ Chinese Med, Coll Clin Med 2, Lab Chinese Mat Med Preparat, Guangdong Prov Inst TCM, Guangzhou, Guangdong, Peoples R China
推荐引用方式(GB/T 7714)：
Xu Wen,Zhang Jing,Huang Zhihai,等.Identification of new dianthrone glycosides from Polygonum multiflorum Thunb. using high-performance liquid chromatography coupled with LTQ-Orbitrap mass spectrometry detection: a strategy for the rapid detection of new low abundant metabolites from traditional Chinese medicines[J].ANALYTICAL METHODS.2012,4(6):1806-1812.
