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LDL Coating pVEGF/polyethylenimine Complex Enhances Vascular Endothelial Growth Factor Expression

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收录情况: ◇ SCIE

机构: [1] Guangdong Prov TCM Hosp, Cardiovasc Lab, Guangzhou 510006, Guangdong, Peoples R China [2] Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Guangdong, Peoples R China
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摘要:
The major limitations to non-viral gene delivery are relatively low efficiency and cytotoxicity, which need to be addressed in the design of new vectors. In this study, negatively charged low density lipoproteins (LDL) were coated onto positively charged pVEGF/PEI complexes to form pVEGF/PEI/LDL terplexes by a two-step procedure. The biocompatible LDL was introduced to reduce the cytotoxicity of the gene delivery system and increase its affinity to cells. The successful formation of pVEGF/PEI/LDL terplexes was confirmed by their near-neutral and slightly negative surface charges. The pVEGF/PEI/LDL terplexes were well-defined sub-micron spherical particles. On the cell viability assay, both of the PEI/LDL combined vector and pVEGF/PEI/LDL terplexes exhibited much lower cytotoxicity to HeLa cells and HUVE cells than those of PEI and pVEGF/PEI complexes, attributed to the shielding effect of the LDL. pEGFP/PEI/LDL terplexes showed significantly higher transfection efficiency in comparison to pEGFP/PEI complexes in serum-containing medium. pVEGF/PEI/LDL terplexes at their optimal N/P ratio and LDL/PEI weigh ratio induced higher expression levels of VEGF protein in HUVE cells than those of pVEGF/PEI complexes. Therefore, the pVEGF/PEI/LDL terplexes could be used as a promising gene delivery system to enhance VEGF protein expression.

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中科院(CAS)分区:
出版当年[2011]版:
大类 | 3 区 工程技术
小类 | 4 区 生物工程与应用微生物
最新[2025]版:
大类 | 4 区 生物学
小类 | 4 区 生物工程与应用微生物
JCR分区:
出版当年[2010]版:
Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
最新[2023]版:
Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

影响因子: 最新[2023版] 最新五年平均 出版当年[2010版] 出版当年五年平均 出版前一年[2009版] 出版后一年[2011版]

第一作者:
第一作者机构: [1] Guangdong Prov TCM Hosp, Cardiovasc Lab, Guangzhou 510006, Guangdong, Peoples R China [*1]Guangdong Prov TCM Hosp, Cardiovasc Lab, Guangzhou 510006, Guangdong, Peoples R China
通讯作者:
通讯机构: [1] Guangdong Prov TCM Hosp, Cardiovasc Lab, Guangzhou 510006, Guangdong, Peoples R China [*1]Guangdong Prov TCM Hosp, Cardiovasc Lab, Guangzhou 510006, Guangdong, Peoples R China
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