机构：[1]Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China[2]The Tradition Chinese Hospital of Guangdong Province; Guangzhou 510120, China
In mammalian cells, stress-induced expression of heat shock protein is controlled by heat shock factor 1 (HSF1). However, HSF1 functions as a regulator of additional genes. In this study, we observed that heat treatment effectively induced expression of Fas. Using bioinformatics, a high affinity and functional HSF1-binding element within the -1996/-1985 oligonucleotide of the 5'-flanking region of the Fas gene was found, and was determined by electrophoretic mobility shift assay and chromatin immunoprecipitation assay. Exogenous expression of a constitutively activative HSF1, induced Fas gene transcription and protein synthesis in the absence of heat stress. Moreover, RNA interference-mediated HSF1 gene-silencing attenuated Fas expression in a heat-induced model. Our results suggested that HSF1 is an important transcription factor of Fas gene.
基金：
This study is supported by the Young Scientist Fund of the
National Natural Science Fund of China (Grant No. 30600274)
and the Guangzhou Science and Technology Board of China
(Grant No. 2006F1-C0171).
语种：
外文
中科院(CAS)分区：
出版当年[2009]版：
大类|1 区生物4 区生物
小类|1 区生化与分子生物学2 区细胞生物学4 区生化与分子生物学4 区细胞生物学
最新[2025]版：
大类|1 区生物学3 区生物学
小类|1 区生化与分子生物学1 区细胞生物学3 区生化与分子生物学3 区细胞生物学
第一作者：
第一作者机构：[2]The Tradition Chinese Hospital of Guangdong Province; Guangzhou 510120, China
通讯作者：
推荐引用方式(GB/T 7714)：
Shunmei E.,Yuanbo Zhao,Yunhong Huang,et al.Heat shock factor 1 is a transcription factor of Fas gene[J].Mol Cell.2010,29(5):527-531.doi:10.1007/s10059-010-0065-4.
APA：
Shunmei E.,Yuanbo Zhao,Yunhong Huang,Kun Lai,Cha Chen...&Jiangying Zou.(2010).Heat shock factor 1 is a transcription factor of Fas gene.Mol Cell,29,(5)
MLA：
Shunmei E.,et al."Heat shock factor 1 is a transcription factor of Fas gene".Mol Cell 29..5(2010):527-531
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