机构:[1]Wuhan University TaiKang Medical School (School of Basic Medical Sciences), Key Laboratory of Combinatorial Biosynthesis and Drug Discovery of Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan, 430071, China[2]State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing, 102206, China[3]Medical School of Guizhou University, Guiyang, 550025, China[4]Anhui Medical University, Hefei, 230032, China[5]Second Clinical Medicine Collage, Guangzhou University Chinese Medicine, Guangzhou, 510006, China
The staging of liver fibrosis is crucial for its early detection and treatment. However, there is still lacking of effective method for the diagnosis of early liver fibrosis. Extracellular vesicles have shown great po-tential in disease diagnosis due to its specific role in cell-cell communication. Here we analyzed the serum extracellular vesicles proteome from fibrotic mice in a data independent acquisition manner. Totally, we identified 869 proteins commonly present in the serum samples. After systematical data filtering for differentially expressed proteins, the potential markers for the diagnosis of early liver fibrosis were obtained. Six vital differentially expressed proteins (HSPA8, EEF2, EEF1A1, PLG, SERPINC1, HRG) were further verified as a potential candidate biomarker panel, which could discriminate early-stage fibrosis mice from normal mice. This is the first time to differentiate early-stage liver fibrosis with serum extracellular vesicles proteome and its derived marker molecules.(c) 2022 Elsevier B.V. All rights reserved.
基金:
National Key R&D Program of China [2017YFA0505100, 2017YFA0505700]; National Natural Science Foundation of China [32141003, 31901037, 31870824, 91839302, 32071431, 32070668]; Beijing-Tianjin-Hebei Basic Research Cooperation Project [J200001]; CAMS Innovation Fund for Medical Sciences [2019-I2M-5e017]; Innovation Foundation of Medicine [20SWAQX34, 19SWAQ17, AWS17J008]; Foundation of State Key Lab of Proteomics [SKLP-K201704, SKLP-K201901]; Mass Spectrometry Platform Open Project of National Center for Protein Sciences Beijing
第一作者机构:[1]Wuhan University TaiKang Medical School (School of Basic Medical Sciences), Key Laboratory of Combinatorial Biosynthesis and Drug Discovery of Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan, 430071, China[2]State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing, 102206, China
通讯作者:
通讯机构:[1]Wuhan University TaiKang Medical School (School of Basic Medical Sciences), Key Laboratory of Combinatorial Biosynthesis and Drug Discovery of Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan, 430071, China[2]State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing, 102206, China[3]Medical School of Guizhou University, Guiyang, 550025, China[4]Anhui Medical University, Hefei, 230032, China[5]Second Clinical Medicine Collage, Guangzhou University Chinese Medicine, Guangzhou, 510006, China[*1]38 Science Park Road, National Center for Protein Sciences (Beijing), Changping District, Beijing, 102206, China[*2]185 East Lake Road, Wuhan, 430071, China
推荐引用方式(GB/T 7714):
Liu Jinfang,Zuo Tao,Zhao Liping,et al.Serum extracellular vesicle proteome differentiate early stage liver fibrosis[J].INTERNATIONAL JOURNAL OF MASS SPECTROMETRY.2022,482:doi:10.1016/j.ijms.2022.116936.
APA:
Liu, Jinfang,Zuo, Tao,Zhao, Liping,Li, Yanchang,Chang, Lei...&Xu, Ping.(2022).Serum extracellular vesicle proteome differentiate early stage liver fibrosis.INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,482,
MLA:
Liu, Jinfang,et al."Serum extracellular vesicle proteome differentiate early stage liver fibrosis".INTERNATIONAL JOURNAL OF MASS SPECTROMETRY 482.(2022)