机构：[1]Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, Nanning, China,[2]Key Laboratory of Clinical Laboratory Medicine of Guangxi Department of Education, Guangxi Medical University, Nanning, China,[3]Department of Gastroenterology, Meizhou People’s Hospital, Meizhou, China,[4]Department of Laboratory Medicine and Guangdong Engineering and Technology Research Center for Rapid Diagnostic Biosensors, Nanfang Hospital, Southern Medical University, Guangzhou, China,[5]Key Laboratory of Bioactive Materials, State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Functional Polymer Ministry of Education, and College of Life Sciences, Nankai University, Tianjin, China,[6]Guangxi Key Laboratory of Thalassemia Research, Guangxi Medical University, Nanning, China,[7]Department of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China大德路总院检验科大德路总院检验科广东省中医院
Rapid and accurate sensing of beta-galactosidase (beta-gal) activity is particularly critical for the early detection of many diseases and has become a topic of interest in recent years. However, most traditional probes for beta-gal sensing often suffer from the disadvantages of narrow dynamic range, low reaction efficiency and are only employed with either colorimetric or fluorescence sensing. Furthermore, beta-galactosidase sensing based assay for efficient detection and antibiotic resistance analysis of Escherichia coli (E.coli) is not available. Here, an enzyme-induced probe assay was reported for dual sensitive fluorescence and colorimetric measurement of beta-gal activity, and was further employed for detection of Escherichia coli and their antibiotic resistance analysis. The DCM-beta gal probe was virtually non-emissive in aqueous solution, while it could be activated by beta-gal to produce bright emission. Under optimized conditions, DCM-beta gal displayed high sensitivity, selectivity and rapid response to beta-gal with a low detection limit of 1.5 x 10(-3) U ml(-1). Importantly, this assay was successfully applied to sensitive detection of E. coli cells with a fast detection process within 5 h and a low detection concentration of 1 x 10(3) CFU ml(-1). Furthermore, the enzyme-activatable assay was also successfully applied for high throughput E. coli antibiotic resistance analysis. The DCM-beta gal strategy is applied for the first time on the detection of E. coli cells and their antibiotic resistance analysis. It is provided with the advantages of high selectively, a simple operation, low cost and rapid detection. The detection platform can also be extended to analyze the level of beta-gal in other types of cells or biological samples. Overall, the simple, effective and dual-readout assay holds promise for efficient sensing of beta-gal activity and provides a potential tool for E. coli detection and their antibiotic resistance analysis.
基金：
This study was supported by the Open Project of NHC Key
Laboratory of Thalassemia Medicine (GJWJWDP202203).
第一作者机构：[1]Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, Nanning, China,[2]Key Laboratory of Clinical Laboratory Medicine of Guangxi Department of Education, Guangxi Medical University, Nanning, China,
共同第一作者：
通讯作者：
通讯机构：[1]Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, Nanning, China,[2]Key Laboratory of Clinical Laboratory Medicine of Guangxi Department of Education, Guangxi Medical University, Nanning, China,
推荐引用方式(GB/T 7714)：
Huang Yifang,Feng Weiwei,Zhang Guo-Qiang,et al.An enzyme-activatable dual-readout probe for sensitive β-galactosidase sensing and Escherichia coli analysis[J].FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY.2022,10:doi:10.3389/fbioe.2022.1052801.
APA：
Huang, Yifang,Feng, Weiwei,Zhang, Guo-Qiang,Qiu, Yuling,Li, Linlin...&Cao, Nannan.(2022).An enzyme-activatable dual-readout probe for sensitive β-galactosidase sensing and Escherichia coli analysis.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,10,
MLA：
Huang, Yifang,et al."An enzyme-activatable dual-readout probe for sensitive β-galactosidase sensing and Escherichia coli analysis".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 10.(2022)
